ArticleNucleic acids research2015
The Krüppel-associated box repressor domain induces reversible and irreversible regulation of endogenous mouse genes by mediating different chromatin states.
Article in Nucleic acids research, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 26 citations in OpenAlex.
- Development of compact transcriptional effectors using high-throughput measurements in diverse contexts.Nature biotechnology · 2025Article
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- Orthogonal transcriptional modulation and gene editing using multiple CRISPR-Cas systems.Molecular therapy : the journal of the American Society of Gene Therapy · 2025Article
- Fluorescent Reporter Systems to Investigate Chromatin Effector Proteins in Living Cells.Methods in molecular biology (Clifton, N.J.) · 2024Article
- SpecificMolecular therapy. Nucleic acids · 2023Article
- Downregulation of Bdnf Expression in Adult Mice Causes Body Weight Gain.Neurochemical research · 2022Article
- Epigenetic repression of gonadotropin gene expression via a GnRH-mediated DNA delivery system.Gene therapy · 2022Article
- What sequencing technologies can teach us about innate immunity.Immunological reviews · 2022Review
- A bipartite element with allele-specific functions safeguards DNA methylation imprints at the Dlk1-Dio3 locus.Developmental cell · 2021Article
- An efficient KRAB domain for CRISPRi applications in human cells.Nature methods · 2020Article
- KRAB-Induced Heterochromatin Effectively SilencesInternational journal of molecular sciences · 2020Article
- The how and why of lncRNA function: An innate immune perspective.Biochimica et biophysica acta. Gene regulatory mechanisms · 2020Review
- Genome Editing for CNS Disorders.Frontiers in neuroscience · 2020Review
- DNA methylation and de-methylation using hybrid site-targeting proteins.Genome biology · 2018Review
- Trithorax dependent changes in chromatin landscape at enhancer and promoter regions drive female puberty.Nature communications · 2018Article
- TRIM28 and Interacting KRAB-ZNFs Control Self-Renewal of Human Pluripotent Stem Cells through Epigenetic Repression of Pro-differentiation Genes.Stem cell reports · 2017Article
- Determination of the physiological and pathological roles of E2F3 in adult tissues.Scientific reports · 2017Article
- CRISPR-Mediated Epigenome Editing.The Yale journal of biology and medicine · 2016Review
- Epigenetic Editing: On the Verge of Reprogramming Gene Expression at Will.Current genetic medicine reports · 2016Review
- Simultaneous live imaging of the transcription and nuclear position of specific genes.Nucleic acids research · 2015Article
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Krüppel-associated box (KRAB) domain is a transcription repression module from the largest family of transcriptional regulators encoded by higher vertebrates. We developed a drug-controllable regulation system based on an artificial KRAB-containing repressor (tTS) that targets the endogenous Hprt gene to explore the regulatory mechanism and molecular basis of KRAB-containing regulators within the context of an endogenous gene in vivo. We show that KRAB can mediate irreversible and reversible regulation of endogenous genes in mouse that is dependent on embryonic developmental stage. KRAB-induced stable DNA methylation within the KRAB binding region during the early embryonic stage, resulting in irreversible gene repression. In later stages, KRAB mainly induced de-acetylation and methylation of histone, resulting in reversible gene repression. Thus, we have characterized the KRAB-mediated regulation system within the context of an endogenous gene and multiple spatiotemporal ranges, thereby providing a basis for identifying the function of KRAB-containing regulators and aiding development of novel KRAB-based gene regulation tools in vivo.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.