ArticleMolecular systems biology2015
Essential gene disruptions reveal complex relationships between phenotypic robustness, pleiotropy, and fitness.
Article in Molecular systems biology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
31 citing papers in PubMed.
- Loss of U11/U12 spliceosome geneLife science alliance · 2026Article
- Molecular evolution in light of regulatory-coding epistasis.EMBO reports · 2026Review
- Systematic data analysis pipeline for quantitative morphological cell phenotyping.Computational and structural biotechnology journal · 2024Review
- Selection for robust metabolism in domesticated yeasts is driven by adaptation to Hsp90 stress.Science (New York, N.Y.) · 2024Article
- A continuous epistasis model for predicting growth rate given combinatorial variation in gene expression and environment.Cell systems · 2024Article
- Application of unimodal probability distribution models for morphological phenotyping of budding yeast.FEMS yeast research · 2024Article
- Genic constraint against nonsynonymous variation across the mouse genome.BMC genomics · 2023Article
- PANTOTHENATE KINASE4, LOSS OF GDU2, and TRANSPOSON PROTEIN1 affect the canalization of tomato fruit metabolism.Plant physiology · 2023Article
- Gene loss and compensatory evolution promotes the emergence of morphological novelties in budding yeast.Nature ecology & evolution · 2022Article
- Computational Study on the Dynamics of Mycobacterium Tuberculosis RNA Polymerase Assembly.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Virulence Factors and in-Host Selection on Phenotypes in Infectious Probiotic Yeast Isolates (Journal of fungi (Basel, Switzerland) · 2021Article
- A simplified strategy for titrating gene expression reveals new relationships between genotype, environment, and bacterial growth.Nucleic acids research · 2021Article
- Phenotypic Robustness of Epidermal Stem Cell Number inFrontiers in cell and developmental biology · 2021Article
- Mismatch-CRISPRi Reveals the Co-varying Expression-Fitness Relationships of Essential Genes in Escherichia coli and Bacillus subtilis.Cell systems · 2020Article
- Genetic buffering and potentiation in metabolism.PLoS computational biology · 2020Article
- Extent and context dependence of pleiotropy revealed by high-throughput single-cell phenotyping.PLoS biology · 2020Article
- SNPeffect: identifying functional roles of SNPs using metabolic networks.The Plant journal : for cell and molecular biology · 2020Article
- Titrating gene expression using libraries of systematically attenuated CRISPR guide RNAs.Nature biotechnology · 2020Article
- Large-effect flowering time mutations reveal conditionally adaptive paths through fitness landscapes inProceedings of the National Academy of Sciences of the United States of America · 2019Article
- Yeast genetic interaction screens in the age of CRISPR/Cas.Current genetics · 2019Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
Abstract
The concept of robustness in biology has gained much attention recently, but a mechanistic understanding of how genetic networks regulate phenotypic variation has remained elusive. One approach to understand the genetic architecture of variability has been to analyze dispensable gene deletions in model organisms; however, the most important genes cannot be deleted. Here, we have utilized two systems in yeast whereby essential genes have been altered to reduce expression. Using high-throughput microscopy and image analysis, we have characterized a large number of morphological phenotypes, and their associated variation, for the majority of essential genes in yeast. Our results indicate that phenotypic robustness is more highly dependent upon the expression of essential genes than on the presence of dispensable genes. Morphological robustness appears to be a general property of a genotype that is closely related to pleiotropy. While the fitness profile across a range of expression levels is idiosyncratic to each gene, the global pattern indicates that there is a window in which phenotypic variation can be released before fitness effects are observable.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.