Evidence map›Paper›PMID 25609542›Full record

ArticleScientific reports2015

Magnetic-activated cell sorting (MACS) can be used as a large-scale method for establishing zebrafish neuronal cell cultures.

Georg Welzel, Daniel Seitz, Stefan Schuster

Open access · goldAbstract read
In one paragraph

Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.

0numbers the graph read from it
0cells of the map it votes in
24citing papers in PubMed
2.0field-weighted citation impact, top 15% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

24 citing papers in PubMed, 45 citations in OpenAlex.

  1. Article
  2. Advanced Microfluidics for Single Cell-Based Cancer Research.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025
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  3. Dynamic and precise electromagnetic levitation of single cells.Proceedings of the National Academy of Sciences of the United States of America · 2025
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  7. An Overview of Advances in Rare Cancer Diagnosis and Treatment.International journal of molecular sciences · 2024
    Review
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  9. Tumor regression efficacy ofBioinformation · 2023
    Article
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  11. Review
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  13. Article
  14. Review
  15. Article
  16. Article
  17. Single-Cell Library Preparation of iPSC-Derived Neural Stem Cells.Methods in molecular biology (Clifton, N.J.) · 2019
    Article
  18. Article
  19. Single Cell Neurometabolomics.ACS chemical neuroscience · 2018
    Review
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Georg Welzel1] Department of Animal Physiology, University of Bayreuth, 95440 Bayreuth, Germany [2] Friedrich-Baur BioMed Center, 95448 Bayreuth.
Daniel Seitz1] Department of Animal Physiology, University of Bayreuth, 95440 Bayreuth, Germany [2] Friedrich-Baur BioMed Center, 95448 Bayreuth.
Stefan Schuster1] Department of Animal Physiology, University of Bayreuth, 95440 Bayreuth, Germany [2] Friedrich-Baur BioMed Center, 95448 Bayreuth.
University of Bayreuth · DE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Neuronal cell cultures offer a crucial tool to mechanistically analyse regeneration in the nervous system. Despite the increasing importance of zebrafish (Danio rerio) as an in vivo model in neurobiological and biomedical research, in vitro approaches to the nervous system are lagging far behind and no method is currently available for establishing enriched neuronal cell cultures. Here we show that magnetic-activated cell sorting (MACS) can be used for the large-scale generation of neuronal-restricted progenitor (NRP) cultures from embryonic zebrafish. Our findings provide a simple and semi-automated method that is likely to boost the use of neuronal cell cultures as a tool for the mechanistic dissection of key processes in neuronal regeneration and development.

Indexed as

AnimalsCell AggregationCell DifferentiationCells, CulturedCell SeparationEmbryo, NonmammalianFlow CytometryNerve NetNeural Stem CellsNeuronsTretinoinZebrafishZebrafish ProteinsTretinoinZebrafish Proteins

Identifiers

PMID25609542
PMCPMC4302367
OpenAlexW1991457184

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.