ArticleLab on a chip2015
An automated programmable platform enabling multiplex dynamic stimuli delivery and cellular response monitoring for high-throughput suspension single-cell signaling studies.
Article in Lab on a chip, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- Self-Regulated Selective Surface Coating Enables Confinement of Adherent Cells in Closed Microfluidic Arrays.Analytical chemistry · 2025Article
- L-2L ladder digital-to-analogue converter for dynamics generation of chemical concentrations.Royal Society open science · 2023Article
- Live imaging of apoptotic signaling flow using tunable combinatorial FRET-based bioprobes for cell population analysis of caspase cascades.Scientific reports · 2022Article
- Modeling immune cell behavior across scales in cancer.Wiley interdisciplinary reviews. Systems biology and medicine · 2020Review
- A System for Analog Control of Cell Culture Dynamics to Reveal Capabilities of Signaling Networks.iScience · 2019Article
- Transport of dynamic biochemical signals in a microfluidic single cell trapping channel with varying cross-sections.The European physical journal. E, Soft matter · 2019Article
- A Microfluidic Fluorescent Flow Cytometry Capable of Quantifying Cell Sizes and Numbers of Specific Cytosolic Proteins.Scientific reports · 2018Article
- A programmable platform for sub-second multichemical dynamic stimulation and neuronal functional imaging in C. elegans.Lab on a chip · 2018Article
- Single-cell resolution of intracellular T cell CaIntegrative biology : quantitative biosciences from nano to macro · 2017Article
- New insights into mammalian signaling pathways using microfluidic pulsatile inputs and mathematical modeling.Integrative biology : quantitative biosciences from nano to macro · 2017Review
- Microfluidic techniques for high throughput single cell analysis.Current opinion in biotechnology · 2016Review
- Three-dimensional models for studying development and disease: moving on from organisms to organs-on-a-chip and organoids.Integrative biology : quantitative biosciences from nano to macro · 2016Review
- Microfluidics in systems biology-hype or truly useful?Current opinion in biotechnology · 2016Review
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
Cell signaling events are orchestrated by dynamic external biochemical cues. By rapidly perturbing cells with dynamic inputs and examining the output from these systems, one could study the structure and dynamic properties of a cellular signaling network. Conventional experimental techniques limit the implementation of these systematic approaches due to the lack of sophistication in manipulating individual cells and the fluid microenvironment around them; existing microfluidic technologies thus far are mainly targeting adherent cells. In this paper we present an automated platform to interrogate suspension cells with dynamic stimuli while simultaneously monitoring cellular responses in a high-throughput manner at single-cell resolution. We demonstrate the use of this platform in an experiment to measure Jurkat T cells in response to distinct dynamic patterns of stimuli; we find cells exhibit highly heterogeneous responses under each stimulation condition. More interestingly, these cells act as low-pass filters, only entrained to the low frequency stimulus signals. We also demonstrate that this platform can be easily programmed to actively generate arbitrary dynamic signals. We envision our platform to be useful in other contexts to study cellular signaling dynamics, which may be difficult using conventional experimental methods.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.