ArticleScientific reports2015
In vitro expansion of corneal endothelial cells on biomimetic substrates.
Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
46 citing papers in PubMed, 1 synthesis or guideline pooled it, 0 citations in OpenAlex.
- Advances in culture, expansion and mechanistic studies of corneal endothelial cells: a systematic review.Journal of biomedical science · 2019Pooled it
- Divergence Between Marker Expression and Phenotype During Human Corneal Endothelial Cell Expansion.Investigative ophthalmology & visual science · 2026Article
- An Optimized Mechanical Peeling Protocol for Mouse Corneal Endothelium.Bioengineering (Basel, Switzerland) · 2026Article
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- Current and Future Cornea Chip Models for Advancing Ophthalmic Research and Therapeutics.Advanced biology · 2025Review
- Critical Design Parameters of Tantalum-Based Comb Structures to Manipulate Mammalian Cell Morphology.Materials (Basel, Switzerland) · 2025Article
- Comparison of the structural integrity and quality of corneal endothelium stored in organ culture storage medium versus Eusol-C.Cell and tissue banking · 2024Article
- Revolutionizing neurotherapeutics: blood-brain barrier-on-a-chip technologies for precise drug delivery.Annals of medicine and surgery (2012) · 2024Review
- A digoxin derivative that potently reduces intraocular pressure: efficacy and mechanism of action in different animal models.American journal of physiology. Cell physiology · 2024Article
- Fabrication of bioengineered corneal endothelial grafts using an allogeneic cornea-derived matrix.Materials today. Bio · 2024Review
- Femtosecond Laser Cutting of Human Crystalline Lens Capsule and Decellularization for Corneal Endothelial Bioengineering.Bioengineering (Basel, Switzerland) · 2024Article
- The time dependent influence of curvature and topography of biomaterials in the behavior of corneal endothelial cells.Frontiers in bioengineering and biotechnology · 2024Article
- Article
- The soil and the seed: The relationship between Descemet's membrane and the corneal endothelium.Experimental eye research · 2023Review
- Biomaterials used for tissue engineering of barrier-forming cell monolayers in the eye.Frontiers in bioengineering and biotechnology · 2023Review
- Unraveling the mechanobiology of cornea: From bench side to the clinic.Frontiers in bioengineering and biotechnology · 2022Review
- In vivo engraftment into the cornea endothelium using extracellular matrix shrink-wrapped cells.Communications materials · 2022Article
- Mechanical Memory Impairs Adipose-Derived Stem Cell (ASC) Adipogenic Capacity After Long-TermCellular and molecular bioengineering · 2021Article
- Optimization of polycaprolactone - based nanofiber matrices for the cultivation of corneal endothelial cells.Scientific reports · 2021Article
- Challenges in corneal endothelial cell culture.Regenerative medicine · 2021Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Corneal endothelial (CE) cells do not divide in vivo, leading to edema, corneal clouding and vision loss when the density drops below a critical level. The endothelium can be replaced by transplanting allogeneic tissue; however, access to donated tissue is limited worldwide resulting in critical need for new sources of corneal grafts. In vitro expansion of CE cells is a potential solution, but is challenging due to limited proliferation and loss of phenotype in vitro via endothelial to mesenchymal transformation (EMT) and senescence. We hypothesized that a bioengineered substrate recapitulating chemo-mechanical properties of Descemet's membrane would improve the in vitro expansion of CE cells while maintaining phenotype. Results show that bovine CE cells cultured on a polydimethylsiloxane surface with elastic modulus of 50 kPa and collagen IV coating achieved >3000-fold expansion. Cells grew in higher-density monolayers with polygonal morphology and ZO-1 localization at cell-cell junctions in contrast to control cells on polystyrene that lost these phenotypic markers coupled with increased α-smooth muscle actin expression and fibronectin fibril assembly. In total, these results demonstrate that a biomimetic substrate presenting native basement membrane ECM proteins and mechanical environment may be a key element in bioengineering functional CE layers for potential therapeutic applications.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.