ArticleBMC genomics2015
Transcriptome analysis reveals in vitro cultured Withania somnifera leaf and root tissues as a promising source for targeted withanolide biosynthesis.
Article in BMC genomics, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Comprehensive safety evaluation ofFrontiers in nutrition · 2025Article
- Current insights into transcriptional role(s) for the nutraceuticalFrontiers in nutrition · 2024Review
- Transcriptomic profiling reveals MEP pathway contributing to ginsenoside biosynthesis in Panax ginseng.BMC genomics · 2019Article
- Innate endophytic fungus, Aspergillus terreus as biotic elicitor of withanolide A in root cell suspension cultures of Withania somnifera.Molecular biology reports · 2019Article
- Third DWF1 paralog in Solanaceae, sterol ΔProceedings of the National Academy of Sciences of the United States of America · 2018Article
- Endophytes of Withania somnifera modulate in planta content and the site of withanolide biosynthesis.Scientific reports · 2018Article
- Transcriptome analysis reveals in vitro-cultured regeneration bulbs as a promising source for targeted3 Biotech · 2018Article
- Article
- Withaferin A inhibits the proliferation of gastric cancer cells by inducing G2/M cell cycle arrest and apoptosis.Oncology letters · 2017Article
- Comprehensive assessment of the genes involved in withanolide biosynthesis from Withania somnifera: chemotype-specific and elicitor-responsive expression.Functional & integrative genomics · 2017Article
- Review
- Review
- Silencing of sterol glycosyltransferases modulates the withanolide biosynthesis and leads to compromised basal immunity of Withania somnifera.Scientific reports · 2016Article
- Article
- Comparative Characterization of the Leaf Tissue ofFrontiers in plant science · 2016Article
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Authors and funding
15 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe production of metabolites via in vitro culture is promoted by the availability of fully defined metabolic pathways. Withanolides, the major bioactive phytochemicals of Withania somnifera, have been well studied for their pharmacological activities. However, only a few attempts have been made to identify key candidate genes involved in withanolide biosynthesis. Understanding the steps involved in withanolide biosynthesis is essential for metabolic engineering of this plant to increase withanolide production.
resultsTranscriptome sequencing was performed on in vitro adventitious root and leaf tissues using the Illumina platform. We obtained a total of 177,156 assembled transcripts with an average unigene length of 1,033 bp. About 13% of the transcripts were unique to in vitro adventitious roots but no unique transcripts were observed in in vitro-grown leaves. A putative withanolide biosynthetic pathway was deduced by mapping the assembled transcripts to the KEGG database, and the expression of candidate withanolide biosynthesis genes -were validated by qRT PCR. The accumulation pattern of withaferin A and withanolide A varied according to the type of tissue and the culture period. Further, we demonstrated that in vitro leaf extracts exhibit anticancer activity against human gastric adenocarcinoma cell lines at sub G1 phase.
conclusionsWe report here a validated large-scale transcriptome data set and the potential biological activity of in vitro cultures of W. somnifera. This study provides important information to enhance tissue-specific expression and accumulation of secondary metabolites, paving the way for industrialization of in vitro cultures of W. somnifera.
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