ArticlePloS one2015
Dynamic growth and shrinkage govern the pH dependence of RecA filament stability.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
What it found
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
7 citing papers in PubMed.
- How strand exchange protein function benefits from ATP hydrolysis.Current opinion in genetics & development · 2021Review
- The regulation mechanism of the C-terminus of RecA proteins during DNA strand-exchange process.Biophysical journal · 2021Article
- Single Molecule Study of the Polymerization of RecA on dsDNA: The Dynamics of Individual Domains.Frontiers in molecular biosciences · 2021Article
- Antibiotic-induced DNA damage results in a controlled loss of pH homeostasis and genome instability.Scientific reports · 2020Article
- Modular microfluidics enables kinetic insight from time-resolved cryo-EM.Nature communications · 2020Article
- ATPase activity tightly regulates RecA nucleofilaments to promote homologous recombination.Cell discovery · 2017Article
- RecA: Regulation and Mechanism of a Molecular Search Engine.Trends in biochemical sciences · 2016Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
Abstract
RecA proteins form a long stable filament on a single-stranded DNA and catalyze strand exchange reaction. The stability of RecA filament changes dramatically with pH, yet its detailed mechanism is not known. Here, using a single molecule assay, we determined the binding and dissociation rates of RecA monomers at the filament ends at various pH. The pH-induced rate changes were moderate but occurred in opposite directions for binding and dissociation, resulting in a substantial increase in filament stability in lower pH. The highly charged residues in C-terminal domain do not contribute to the pH dependent stability. The stability enhancement of RecA filament in low pH may help the cell to cope with acidic stress by fine-tuning of the binding and dissociation rates without losing the highly dynamic nature of the filament required for strand exchange.
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Registered trials
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