ArticleNature communications2015
Sequencing of first-strand cDNA library reveals full-length transcriptomes.
Article in Nature communications, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Optimized MT-Capture targeted enrichment sequencing for the complete genome of norovirus in stool and sewage samples.Frontiers in microbiology · 2026Article
- Biomolecules Interacting with Long Noncoding RNAs.Biology · 2025Review
- Coordinated neuron-specific splicing events restrict nucleosome engagement of the LSD1 histone demethylase complex.Cell reports · 2025Article
- Article
- Type I/type III IFN and related factors regulate JEV infection and BBB endothelial integrity.Journal of neuroinflammation · 2023Article
- Alcohol-specific transcriptional dynamics of memory reconsolidation and relapse.Translational psychiatry · 2023Article
- Epitranscriptomic mapping of RNA modifications at single-nucleotide resolution using rhodamine sequencing (Rho-seq).STAR protocols · 2022Article
- Sequences to Differences in Gene Expression: Analysis of RNA-Seq Data.Methods in molecular biology (Clifton, N.J.) · 2022Review
- An ultra low-input method for global RNA structure probing uncovers Regnase-1-mediated regulation in macrophages.Fundamental research · 2022Article
- Screening a Spliced Leader-BasedMicroorganisms · 2021Article
- SL-quant: a fast and flexible pipeline to quantify spliced leader trans-splicing events from RNA-seq data.GigaScience · 2018Article
- Capturing the 'ome': the expanding molecular toolbox for RNA and DNA library construction.Nucleic acids research · 2018Review
- Transcriptome Analysis Revealed Impaired cAMP Responsiveness in PHF21A-Deficient Human Cells.Neuroscience · 2018Article
- Altered Gene-Regulatory Function of KDM5C by a Novel Mutation Associated With Autism and Intellectual Disability.Frontiers in molecular neuroscience · 2018Article
- A Mouse Model of X-linked Intellectual Disability Associated with Impaired Removal of Histone Methylation.Cell reports · 2016Article
- Article
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Authors and funding
4 authors.
Funding
Abstract
Massively parallel strand-specific sequencing of RNA (ssRNA-seq) has emerged as a powerful tool for profiling complex transcriptomes. However, many current methods for ssRNA-seq suffer from the underrepresentation of both the 5' and 3' ends of RNAs, which can be attributed to second-strand cDNA synthesis. The 5' and 3' ends of RNA harbour crucial information for gene regulation; namely, transcription start sites (TSSs) and polyadenylation sites. Here we report a novel ssRNA-seq method that does not involve second-strand cDNA synthesis, as we Directly Ligate sequencing Adaptors to the First-strand cDNA (DLAF). This novel method with fewer enzymatic reactions results in a higher quality of the libraries than the conventional method. Sequencing of DLAF libraries followed by a novel analysis pipeline enables the profiling of both 5' ends and polyadenylation sites at near-base resolution. Therefore, DLAF offers the first genomics tool to obtain the 'full-length' transcriptome with a single library.
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