ArticleMeta gene2014
Genetic stability and phytochemical analysis of the in vitro regenerated plants of Dendrobium nobile Lindl., an endangered medicinal orchid.
Article in Meta gene, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 153 citations in OpenAlex.
- Chemical Fingerprinting ofFood science & nutrition · 2026Article
- Preventive effects ofBiomedical reports · 2026Article
- Antioxidant and cytotoxic properties of protocorm-derived phenol-rich fractions of Dendrobium amoenum.BMC complementary medicine and therapies · 2025Article
- Article
- The Transcriptome Profiling of Flavonoids and Bibenzyls Reveals Medicinal Importance of Rare OrchidFrontiers in plant science · 2022Article
- RAPD, ISSR, and SCoT markers based genetic stability assessment of micropropagatedPhysiology and molecular biology of plants : an international journal of functional plant biology · 2021Article
- DNA barcoding and genetic fidelity assessment of micropropagatedPhysiology and molecular biology of plants : an international journal of functional plant biology · 2020Article
- Micropropagation ofPlants (Basel, Switzerland) · 2020Article
- Droplet-vitrification ofBiotechnology reports (Amsterdam, Netherlands) · 2020Article
- Mining of miRNAs from EST data in Dendrobium nobile.Bioinformation · 2020Article
- Phenolic compounds, antioxidant capacity and antimutagenic activity in different growth stages of in vitro raised plants of Origanum vulgare L.Molecular biology reports · 2019Article
- Thidiazuron-induced abnormalities in plant tissue cultures.Plant cell reports · 2018Review
- Review
- Insights into nuclear DNA content, hydrogen peroxide and antioxidative enzyme activities during transverse thin cell layer organogenesis andPhysiology and molecular biology of plants : an international journal of functional plant biology · 2017Article
- Structural Analysis and Immuno-Stimulating Activity of an Acidic Polysaccharide from the Stems of Dendrobium nobile Lindl.Molecules (Basel, Switzerland) · 2017Article
- Phenolic Compounds and Antioxidant Activity of Phalaenopsis Orchid Hybrids.Antioxidants (Basel, Switzerland) · 2016Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
An efficient genetically stable regeneration protocol with increased phytochemical production has been established for Dendrobium nobile, a highly prized orchid for its economic and medicinal importance. Protocorm like bodies (PLBs) were induced from the pseudostem segments using thidiazuron (TDZ; 1.5 mg/l), by-passing the conventional auxin-cytokinin complement approach for plant regeneration. Although, PLB induction was observed at higher concentrations of TDZ, plantlet regeneration from those PLBs was affected adversely. The best rooting (5.41 roots/shoot) was achieved in MS medium with 1.5 mg/l TDZ and 0.25% activated charcoal. Plantlets were successfully transferred to a greenhouse with a survival rate of 84.3%, exhibiting normal development. Genetic stability of the regenerated plants was investigated using randomly amplified polymorphic DNA (RAPD) and start codon targeted (SCoT) polymorphism markers which detected 97% of genetic fidelity among the regenerants. The PIC values of RAPD and SCoT primers were recorded to be 0.92 and 0.76 and their Rp values ranged between 3.66 and 10, and 4 and 12 respectively. The amplification products of the regenerated plants showed similar banding patterns to that of the mother plant thus demonstrating the homogeneity of the micropropagated plants. A comparative phytochemical analysis among the mother and the micropropagated plants showed a higher yield of secondary metabolites. The regeneration protocol developed in this study provides a basis for ex-situ germplasm conservation and also harnesses the various secondary metabolite compounds of medicinal importance present in D. nobile.
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