ArticleMeta gene2014
Designing, optimization and validation of tetra-primer ARMS PCR protocol for genotyping mutations in caprine Fec genes.
Article in Meta gene, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 49 citations in OpenAlex.
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- Factor V Leiden, MTHFR, and FXIIIVal34Leu gene polymorphisms and their association with clinical features and risk of diabetic retinopathy in patients with type 2 diabetes.Caspian journal of internal medicine · 2024Article
- Development of a tetra-primer ARMS-PCR for identification of sika and red deer and their hybrids.Analytical sciences : the international journal of the Japan Society for Analytical Chemistry · 2023Article
- A Simple, Rapid, and Cost-Effective PCR Procedure for Detection ofJournal of laboratory physicians · 2023Article
- Bioinformatics analysis and the association of polymorphisms within the caprineAnimal biotechnology · 2023Article
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- Comprehensive Molecular Evaluation of HNF-1 Alpha, miR-27a, and miR-146 Gene Variants and Their Link with Predisposition and Progression in Type 2 Diabetes Patients.Journal of personalized medicine · 2023Article
- Polymorphisms Analysis ofGenes · 2023Article
- Identification of Rice Accessions Having Cold Tolerance at the Seedling Stage and Development of Novel Genotypic Assays for Predicting Cold Tolerance.Plants (Basel, Switzerland) · 2023Article
- Prevalence ofJournal of obesity · 2023Article
- IdentifyingFood science & nutrition · 2022Article
- Designing, optimization, and validation of whole blood direct T-ARMS PCR for precise and rapid genotyping of complex vertebral malformation in cattle.BMC biotechnology · 2021Article
- A rapid and cost-effective multiplex ARMS-PCR method for the simultaneous genotyping of the circulating SARS-CoV-2 phylogenetic clades.Journal of medical virology · 2021Article
- Genetic Effects of Single Nucleotide Polymorphisms in the GoatAnimals : an open access journal from MDPI · 2019Review
- PCR-based genotyping of SNP markers in sheep.Molecular biology reports · 2018Article
- T-ARMS PCR genotyping of SNP rs445709131 using thermostable strand displacement polymerase.BMC research notes · 2018Article
- Values of molecular markers in the differential diagnosis of thyroid abnormalities.Journal of cancer research and clinical oncology · 2017Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
New, quick, and inexpensive methods for genotyping novel caprine Fec gene polymorphisms through tetra-primer ARMS PCR were developed in the present investigation. Single nucleotide polymorphism (SNP) genotyping needs to be attempted to establish association between the identified mutations and traits of economic importance. In the current study, we have successfully genotyped three new SNPs identified in caprine fecundity genes viz. T(-242)C (BMPR1B), G1189A (GDF9) and G735A (BMP15). Tetra-primer ARMS PCR protocol was optimized and validated for these SNPs with short turn-around time and costs. The optimized techniques were tested on 158 random samples of Black Bengal goat breed. Samples with known genotypes for the described genes, previously tested in duplicate using the sequencing methods, were employed for validation of the assay. Upon validation, complete concordance was observed between the tetra-primer ARMS PCR assays and the sequencing results. These results highlight the ability of tetra-primer ARMS PCR in genotyping of mutations in Fec genes. Any associated SNP could be used to accelerate the improvement of goat reproductive traits by identifying high prolific animals at an early stage of life. Our results provide direct evidence that tetra-primer ARMS-PCR is a rapid, reliable, and cost-effective method for SNP genotyping of mutations in caprine Fec genes.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.