ArticleRNA (New York, N.Y.)2015
A deeply conserved, noncanonical miRNA hosted by ribosomal DNA.
Article in RNA (New York, N.Y.), 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
31 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Computational meta-analysis of ribosomal RNA fragments: potential targets and interaction mechanisms.Nucleic acids research · 2021Pooled it
- A microRNA generated via lysosomal processing of ribosomal RNA suppresses proinflammatory responses.Life science alliance · 2026Article
- Screening for non-invasive rsRNA biomarkers to assess embryo quality utilizing ultra-sensitive pandora sequencing combined with machine learning.Journal of assisted reproduction and genetics · 2025Article
- Repeated emergence of giant microRNA hairpins across invertebrates.Cell reports · 2025Article
- rRFtargetDB: a database of Ago1-mediated targets of ribosomal RNA fragments.RNA (New York, N.Y.) · 2025Article
- Functions of RNAi Pathways in Ribosomal RNA Regulation.Non-coding RNA · 2024Review
- piRNA generation is associated with the pioneer round of translation in stem cells.Nucleic acids research · 2024Article
- Small RNAs: An expanding world with therapeutic promises.Fundamental research · 2023Review
- The Chromosomal Distribution of Sex-Biased MicroRNAs in Drosophila is Nonadaptive.Genome biology and evolution · 2022Article
- Making Invisible RNA Visible: Discriminative Sequencing Methods for RNA Molecules with Specific Terminal Formations.Biomolecules · 2022Review
- Fourth Report on Chicken Genes and Chromosomes 2022.Cytogenetic and genome research · 2022Review
- Small Noncoding RNAs in Reproduction and Infertility.Biomedicines · 2021Review
- Human rDNA and Cancer.Cells · 2021Review
- An essential role for the piRNA pathway in regulating the ribosomal RNA pool in C. elegans.Developmental cell · 2021Article
- Insecticidal RNA interference, thinking beyond long dsRNA.Pest management science · 2021Review
- Three Ribosomal Operons ofFrontiers in microbiology · 2021Article
- Developmentally Regulated Novel Non-coding Anti-sense Regulators of mRNA Translation iniScience · 2020Article
- Article
- Repression of G1/S Transition by Transient Inhibition of miR-10404 Expression in Drosophila Primordial Germ Cells.iScience · 2020Article
- Nuclear Argonaute Piwi Gene Mutation Affects rRNA by Inducing rRNA Fragment Accumulation, Antisense Expression, and Defective Processing inInternational journal of molecular sciences · 2020Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
Advances in small RNA sequencing technologies and comparative genomics have fueled comprehensive microRNA (miRNA) gene annotations in humans and model organisms. Although new miRNAs continue to be discovered in recent years, these have universally been lowly expressed, recently evolved, and of debatable endogenous activity, leading to the general assumption that virtually all biologically important miRNAs have been identified. Here, we analyzed small RNAs that emanate from the highly repetitive rDNA arrays of Drosophila. In addition to endo-siRNAs derived from sense and antisense strands of the pre-rRNA sequence, we unexpectedly identified a novel, deeply conserved, noncanonical miRNA. Although this miRNA is widely expressed, this miRNA was not identified by previous studies due to bioinformatics filters removing such repetitive sequences. Deep-sequencing data provide clear evidence for specific processing with precisely defined 5' and 3' ends. Furthermore, we demonstrate that the mature miRNA species is incorporated in the effector complexes and has detectable trans regulatory activity. Processing of this miRNA requires Dicer-1, whereas the Drosha-Pasha complex is dispensable. The miRNA hairpin sequence is located in the internal transcribed spacer 1 region of rDNA and is highly conserved among Dipteran species that were separated from their common ancestor ∼ 100 million years ago. Our results suggest that biologically active miRNA genes may remain unidentified even in well-studied organisms.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.