ArticleDevelopment (Cambridge, England)2015
A gene expression atlas of a bicoid-depleted Drosophila embryo reveals early canalization of cell fate.
Article in Development (Cambridge, England), 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Zero-shot reconstruction of mutant spatial transcriptomes.Patterns (New York, N.Y.) · 2026Article
- Heat tolerance, oxidative stress response tuning and robust gene activation in early-stageProceedings. Biological sciences · 2024Article
- Revisiting bicoid function: complete inactivation reveals an additional fundamental role in Drosophila egg geometry specification.Hereditas · 2024Article
- Functional analysis of the Drosophila eve locus in response to non-canonical combinations of gap gene expression levels.Developmental cell · 2023Article
- Latent space of a small genetic network: Geometry of dynamics and information.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- A Mutation in theG3 (Bethesda, Md.) · 2020Article
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- An Atlas of Transcription Factors Expressed in Male Pupal Terminalia ofG3 (Bethesda, Md.) · 2019Article
- Quantitative Comparison of the Anterior-Posterior Patterning System in the Embryos of FiveG3 (Bethesda, Md.) · 2019Article
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- Hunchback is counter-repressed to regulate even-skipped stripe 2 expression in Drosophila embryos.PLoS genetics · 2018Article
- Computational Model of Secondary Palate Fusion and Disruption.Chemical research in toxicology · 2017Article
- Genome-wide measurement of spatial expression in patterning mutants ofF1000Research · 2017Article
- Morphogen rules: design principles of gradient-mediated embryo patterning.Development (Cambridge, England) · 2015Review
- Critical Timing without a Timer for Embryonic Development.Biophysical journal · 2015Article
- Krüppel Expression Levels Are Maintained through Compensatory Evolution of Shadow Enhancers.Cell reports · 2015Article
- Article
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6 authors.
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Abstract
In developing embryos, gene regulatory networks drive cells towards discrete terminal fates, a process called canalization. We studied the behavior of the anterior-posterior segmentation network in Drosophila melanogaster embryos by depleting a key maternal input, bicoid (bcd), and measuring gene expression patterns of the network at cellular resolution. This method results in a gene expression atlas containing the levels of mRNA or protein expression of 13 core patterning genes over six time points for every cell of the blastoderm embryo. This is the first cellular resolution dataset of a genetically perturbed Drosophila embryo that captures all cells in 3D. We describe the technical developments required to build this atlas and how the method can be employed and extended by others. We also analyze this novel dataset to characterize the degree and timing of cell fate canalization in the segmentation network. We find that in two layers of this gene regulatory network, following depletion of bcd, individual cells rapidly canalize towards normal cell fates. This result supports the hypothesis that the segmentation network directly canalizes cell fate, rather than an alternative hypothesis whereby cells are initially mis-specified and later eliminated by apoptosis. Our gene expression atlas provides a high resolution picture of a classic perturbation and will enable further computational modeling of canalization and gene regulation in this transcriptional network.
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