ArticleThe Journal of biological chemistry2015
Disruption of a conserved CAP-D3 threonine alters condensin loading on mitotic chromosomes leading to chromosome hypercondensation.
Article in The Journal of biological chemistry, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 27 citations in OpenAlex.
- Regulation of condensin II by self-suppression and release mechanisms.Molecular biology of the cell · 2024Article
- Role and mechanism ofFrontiers in pharmacology · 2024Article
- Regulation of the mitotic chromosome folding machines.The Biochemical journal · 2022Article
- Molecular dissection of condensin II-mediated chromosome assembly using in vitro assays.eLife · 2022Article
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- Condensin II protein dysfunction impacts mitochondrial respiration and mitochondrial oxidative stress responses.Journal of cell science · 2019Article
- Dysregulation of the cohesin subunit RAD21 by Hepatitis C virus mediates host-virus interactions.Nucleic acids research · 2019Article
- Comparing and Contrasting the Effects ofGenetics · 2018Article
- Transcription of a B chromosome CAP-G pseudogene does not influence normal Condensin Complex genes in a grasshopper.Scientific reports · 2017Article
- An SMC-like protein binds and regulates Caenorhabditis elegans condensins.PLoS genetics · 2017Article
- Condensin, master organizer of the genome.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2017Review
- Interdependency and phosphorylation of KIF4 and condensin I are essential for organization of chromosome scaffold.PloS one · 2017Article
- Condensin recruitment to chromatin is inhibited by Chk2 kinase in response to DNA damage.Cell cycle (Georgetown, Tex.) · 2016Article
- The Clathrin-dependent Spindle Proteome.Molecular & cellular proteomics : MCP · 2016Article
- Sister chromatid resolution is an intrinsic part of chromosome organization in prophase.Nature cell biology · 2016Article
- Condensin I and II behaviour in interphase nuclei and cells undergoing premature chromosome condensation.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2016Article
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
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Abstract
The condensin complex plays a key role in organizing mitotic chromosomes. In vertebrates, there are two condensin complexes that have independent and cooperative roles in folding mitotic chromosomes. In this study, we dissect the role of a putative Cdk1 site on the condensin II subunit CAP-D3 in chicken DT40 cells. This conserved site has been shown to activate condensin II during prophase in human cells, and facilitate further phosphorylation by polo-like kinase I. We examined the functional significance of this phosphorylation mark by mutating the orthologous site of CAP-D3 (CAP-D3(T1403A)) in chicken DT40 cells. We show that this mutation is a gain of function mutant in chicken cells; it disrupts prophase, results in a dramatic shortening of the mitotic chromosome axis, and leads to abnormal INCENP localization. Our results imply phosphorylation of CAP-D3 acts to limit condensin II binding onto mitotic chromosomes. We present the first in vivo example that alters the ratio of condensin I:II on mitotic chromosomes. Our results demonstrate this ratio is a critical determinant in shaping mitotic chromosomes.
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