ArticleMethods in enzymology2015
Live-cell imaging of mammalian RNAs with Spinach2.
Article in Methods in enzymology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
14 citing papers in PubMed, 27 citations in OpenAlex.
- Visualizing Diverse RNA Functions in Living Cells With SpinachBio-protocol · 2026Article
- Regulation of Subcellular Protein Synthesis for Restoring Neural Connectivity.International journal of molecular sciences · 2025Review
- Identification of Host Factors Differentially Induced by Clinically Diverse Strains of Tick-Borne Encephalitis Virus.Journal of virology · 2022Article
- Photophysics of DFHBI bound to RNA aptamer Baby Spinach.Scientific reports · 2021Article
- Nucleic-Acid Structures as Intracellular Probes for Live Cells.Advanced materials (Deerfield Beach, Fla.) · 2020Review
- Nanomechanics and co-transcriptional folding of Spinach and Mango.Nature communications · 2019Article
- From "Cellular" RNA to "Smart" RNA: Multiple Roles of RNA in Genome Stability and Beyond.Chemical reviews · 2018Review
- Use of Baby Spinach and Broccoli for imaging of structured cellular RNAs.Nucleic acids research · 2017Article
- Live Cell Imaging of Endogenous mRNA Using RNA-Based Fluorescence "Turn-On" Probe.ACS chemical biology · 2017Article
- Illuminating Messengers: An Update and Outlook on RNA Visualization in Bacteria.Frontiers in microbiology · 2017Review
- A proximity-dependent assay for specific RNA-protein interactions in intact cells.RNA (New York, N.Y.) · 2016Article
- Engineering a ribozyme cleavage-induced split fluorescent aptamer complementation assay.Nucleic acids research · 2016Article
- iSpinach: a fluorogenic RNA aptamer optimized for in vitro applications.Nucleic acids research · 2016Article
- Visual detection of Flavivirus RNA in living cells.Methods (San Diego, Calif.) · 2016Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The ability to monitor RNAs of interest in living cells is crucial to understanding the function, dynamics, and regulation of this important class of molecules. In recent years, numerous strategies have been developed with the goal of imaging individual RNAs of interest in living cells, each with their own advantages and limitations. This chapter provides an overview of current methods of live-cell RNA imaging, including a detailed discussion of genetically encoded strategies for labeling RNAs in mammalian cells. This chapter then focuses on the development and use of "RNA mimics of GFP" or Spinach technology for tagging mammalian RNAs and includes a detailed protocol for imaging 5S and CGG60 RNA with the recently described Spinach2 tag.
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Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.