ArticleBMC plant biology2015
Identification and characterization of microRNAs from Chinese pollination constant non-astringent persimmon using high-throughput sequencing.
Article in BMC plant biology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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20 citing papers in PubMed, 72 citations in OpenAlex.
- Non-coding RNA: a new perspective on the regulation of secondary metabolites in medicinal plants.Molecular horticulture · 2026Review
- MicroRNA-Mediated Hormonal Control of Fruit Morphology.Plants (Basel, Switzerland) · 2026Review
- Construction and Functional Analysis of the ceRNA Regulatory Network Associated with Muscle Development in Shaanbei White Cashmere Goats.Animals : an open access journal from MDPI · 2025Article
- What do microRNA concentrations tell us about the mechanical damage and storage period of strawberry fruits?Food chemistry. Molecular sciences · 2025Article
- Genome-wide identification and expression analysis of Phenylalanine Ammonia-Lyase (PAL) in cucumber.PloS one · 2025Article
- Detection of Ethylene Signaling Using Bioinformatics Tools in Plants.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Machine learning to establish three sphingolipid metabolism genes signature to characterize the immune landscape and prognosis of patients with gastric cancer.BMC genomics · 2024Article
- Coordinating Diverse Functions of miRNA and lncRNA in Fleshy Fruit.Plants (Basel, Switzerland) · 2023Review
- An insight into microRNA biogenesis and its regulatory role in plant secondary metabolism.Plant cell reports · 2022Review
- Identification of microRNAs and their target genes related to the accumulation of anthocyanin in purple potato tubers (Plant direct · 2022Article
- DkmiR397 Regulates Proanthocyanidin Biosynthesis via Negative ModulatingInternational journal of molecular sciences · 2022Article
- Biosynthesis of Secondary Metabolites Based on the Regulation of MicroRNAs.BioMed research international · 2022Review
- Identification of microRNAs from Medicinal PlantPlants (Basel, Switzerland) · 2021Article
- Small RNA sequencing reveals various microRNAs involved in piperine biosynthesis in black pepper (Piper nigrum L.).BMC genomics · 2021Article
- MiR858b Inhibits Proanthocyanidin Accumulation by the Repression ofFrontiers in plant science · 2020Article
- MicroRNA858-mediated regulation of anthocyanin biosynthesis in kiwifruit (Actinidia arguta) based on small RNA sequencing.PloS one · 2019Article
- Small RNA sequencing reveals a role for sugarcane miRNAs and their targets in response to Sporisorium scitamineum infection.BMC genomics · 2017Article
- Contemporary Understanding of miRNA-Based Regulation of Secondary Metabolites Biosynthesis in Plants.Frontiers in plant science · 2017Review
- Application of Genomic Technologies to the Breeding of Trees.Frontiers in genetics · 2016Review
- Identification and characterization of miRNAs in ripening fruit of Lycium barbarum L. using high-throughput sequencing.Frontiers in plant science · 2015Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundmicroRNAs (miRNAs) have been shown to play key roles in regulating gene expression at post-transcriptional level, but miRNAs associated with natural deastringency of Chinese pollination-constant nonastringent persimmon (CPCNA) have never been identified.
resultsIn this study, two small RNA libraries established using 'Eshi No. 1' persimmon (Diospyros kaki Thunb.; CPCNA) fruits collected at 15 and 20 weeks after flowering (WAF) were sequenced through Solexa platform in order to identify miRNAs involved in deastringency of persimmon. A total of 6,258,487 and 7,634,169 reads were generated for the libraries at 15 and 20 WAF, respectively. Based on sequence similarity and hairpin structure prediction, 236 known miRNAs belonging to 65 miRNA families and 33 novel miRNAs were identified using persimmon transcriptome data. Sixty one of the characterized miRNAs exhibited pronounced difference in the expression levels between 15 and 20 WAF, 17 up-regulated and 44 down-regulated. Expression profiles of 12 conserved and 10 novel miRNAs were validated by stem loop qRT-PCR. A total of 198 target genes were predicted for the differentially expressed miRNAs, including several genes that have been reported to be implicated in proanthocyanidins (PAs, or called tannin) accumulation. In addition, two transcription factors, a GRF and a bHLH, were experimentally confirmed as the targets of dka-miR396 and dka-miR395, respectively.
conclusionsTaken together, the present data unraveled several important miRNAs in persimmon. Among them, miR395p-3p and miR858b may regulate bHLH and MYB, respectively, which are influenced by SPL under the control of miR156j-5p and in turn regulate the structural genes involved in PA biosynthesis. In addition, dka-miR396g and miR2911a may regulate their target genes associated with glucosylation and insolubilization of tannin precursors. All of these miRNAs might play key roles in the regulation of (de)astringency in persimmon fruits under normal development conditions.
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