ArticleGenesis (New York, N.Y. : 2000)2015
Generation and characterization of an endothelin-2 iCre mouse.
Article in Genesis (New York, N.Y. : 2000), 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 12 citations in OpenAlex.
- Acute Amelioration of Inflammatory Activity Caused by Endothelin-2 Deficiency during Acute Lung Injury.The Kobe journal of medical sciences · 2023Article
- Progenitor-derived endothelin controls dermal sheath contraction for hair follicle regression.Nature cell biology · 2023Article
- Article
- Endothelin 2: a key player in ovulation and fertility.Reproduction (Cambridge, England) · 2022Review
- Protective Effects of Endothelin-2 Expressed in Epithelial Cells on Bleomycin-Induced Pulmonary Fibrosis in Mice.The Kobe journal of medical sciences · 2021Article
- Characterization of a BAC transgenic mouse expressing Krt19-driven iCre recombinase in its digestive organs.PloS one · 2019Article
- Granulosa cell endothelin-2 expression is fundamental for ovulatory follicle rupture.Scientific reports · 2017Article
- Transgenic and gene knockout mice in gastric cancer research.Oncotarget · 2017Review
- Estradiol Synthesis in Gut-Associated Lymphoid Tissue: Leukocyte Regulation by a Sexually Monomorphic System.Endocrinology · 2016Article
- Core Binding Factor-β Knockdown Alters Ovarian Gene Expression and Function in the Mouse.Molecular endocrinology (Baltimore, Md.) · 2016Article
- Endothelin.Pharmacological reviews · 2016Review
- Generation of an estrogen receptor beta-iCre knock-in mouse.Genesis (New York, N.Y. : 2000) · 2016Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
A novel transgenic mouse line that expresses codon-improved Cre recombinase (iCre) under regulation of the Endothelin-2 gene (edn2) promoter was developed for the conditional deletion of genes in Endothelin-2 lineage cells and for the spatial and temporal localization of Endothelin-2 expression. Endothelin-2 (EDN2, ET-2, previously VIC) is a transcriptionally regulated 21 amino acid peptide implicated in vascular homeostasis, and more recently in female reproduction, gastrointestinal function, immunology, and cancer pathogenesis that acts through membrane receptors and G-protein signaling. A cassette (edn2-iCre) was constructed that contained iCre, a polyadenylation sequence, and a neomycin selection marker in front of the endogenous start codon of the edn2 gene in a mouse genome BAC clone. The cassette was introduced into the C57BL/6 genome by pronuclear injection, and two lines of edn2-iCre positive mice were produced. The edn2-iCre mice were bred with ROSA26-lacZ and Ai9 reporter mice to visualize areas of functional iCre expression. Strong expression was seen in the periovulatory ovary, stomach and small intestine, and colon. Uniquely, we report punctate expression in the corneal epithelium, the liver, the lung, the pituitary, the uterus, and the heart. In the embryo, expression is localized in developing hair follicles and the dermis. Therefore, edn2-iCre mice will serve as a novel line for conditional gene deletion in these tissues.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.