ArticleMarine drugs2015
Enzyme-assisted extraction of bioactive material from Chondrus crispus and Codium fragile and its effect on herpes simplex virus (HSV-1).
Article in Marine drugs, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
29 citing papers in PubMed, 1 synthesis or guideline pooled it, 96 citations in OpenAlex.
- Pooled it
- Bioactive Sulfated Polysaccharides from Green AlgaeInternational journal of molecular sciences · 2026Article
- Bioactive Compounds of Plant-Based Food: Extraction, Isolation, Identification, Characteristics, and Emerging Applications.Food science & nutrition · 2025Review
- Advancing sustainable agriculture: the potential of seaweed-derived bio pesticides from marine biomass.Bioresources and bioprocessing · 2025Review
- Polysaccharides with Arabinose: Key Players in Reducing Chronic Inflammation and Enhancing Immune Health in Aging.Molecules (Basel, Switzerland) · 2025Review
- Recovery of carotenoids as bioactive compounds from peach pomace by an eco-friendly ultrasound-assisted enzymatic extraction.Journal of food science and technology · 2024Article
- From algae to advancements: laminarin in biomedicine.RSC advances · 2024Review
- An Update on the Chemical Constituents and Biological Properties of Selected Species of an Underpinned Genus of Red Algae:Marine drugs · 2024Review
- Anti-SARS-CoV-2 Activity of Polysaccharides Extracted fromMarine drugs · 2023Article
- Macroalgae-Derived Multifunctional Bioactive Substances: The Potential Applications for Food and Pharmaceuticals.Foods (Basel, Switzerland) · 2022Review
- A Critical Comparison of the Advanced Extraction Techniques Applied to Obtain Health-Promoting Compounds from Seaweeds.Marine drugs · 2022Review
- Biological properties and potential of compounds extracted from red seaweeds.Phytochemistry reviews : proceedings of the Phytochemical Society of Europe · 2022Review
- Antiviral Activity and Mechanisms of Seaweeds Bioactive Compounds on Enveloped Viruses-A Review.Marine drugs · 2022Review
- A critical look at challenges and future scopes of bioactive compounds and their incorporations in the food, energy, and pharmaceutical sector.Environmental science and pollution research international · 2022Review
- Advancement of biorefinery-derived platform chemicals from macroalgae: a perspective for bioethanol and lactic acid.Biomass conversion and biorefinery · 2022Review
- Macroalgal Proteins: A Review.Foods (Basel, Switzerland) · 2022Review
- Sulfated Polysaccharides from Seaweed Strandings as Renewable Source for Potential Antivirals againstMarine drugs · 2022Article
- A concise review of the bioactivity and pharmacological properties of the genusJournal of applied phycology · 2022Review
- Characterization and Comparative Analysis of MicroRNAs in 3 Representative Red Algae.Iranian journal of biotechnology · 2021Article
- Dietary supplementation of Ascophylum nodosum improved kidney function of mink challenged with Aleutian mink disease virus.BMC veterinary research · 2020Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Codium fragile and Chondrus crispus are, respectively, green and red seaweeds which are abundant along the North Atlantic coasts. We investigated the chemical composition and antiviral activity of enzymatic extracts of C. fragile (CF) and C. crispus (CC). On a dry weight basis, CF consisted of 11% protein, 31% neutral sugars, 0.8% sulfate, 0.6% uronic acids, and 49% ash, while CC contained 27% protein, 28% neutral sugars, 17% sulfate, 1.8% uronic acids, and 25% ash. Enzyme-assisted hydrolysis improved the extraction efficiency of bioactive materials. Commercial proteases and carbohydrases significantly improved (p ≤ 0.001) biomass yield (40%-70% dry matter) as compared to aqueous extraction (20%-25% dry matter). Moreover, enzymatic hydrolysis enhanced the recovery of protein, neutral sugars, uronic acids, and sulfates. The enzymatic hydrolysates exhibited significant activity against Herpes simplex virus (HSV-1) with EC50 of 77.6-126.8 μg/mL for CC and 36.5-41.3 μg/mL for CF, at a multiplicity of infection (MOI) of 0.001 ID50/cells without cytotoxity (1-200 μg/mL). The extracts obtained from proteases (P1) and carbohydrases (C3) were also effective at higher virus MOI of 0.01 ID50/cells without cytotoxity. Taken together, these results indicate the potential application of enzymatic hydrolysates of C. fragile and C. crispus in functional food and antiviral drug discovery.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.