Evidence map›Paper›PMID 25603302›Full record

ArticleJournal of hazardous materials2015

Absolute quantification of norovirus capsid protein in food, water, and soil using synthetic peptides with electrospray and MALDI mass spectrometry.

Erica M Hartmann, David R Colquhoun, Kellogg J Schwab, Rolf U Halden

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of hazardous materials, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
1.0field-weighted citation impact, top 25% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 12 citations in OpenAlex.

  1. Review
  2. Article
  3. Article
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 2 institutions in 1 country.

Erica M HartmannCenter for Environmental Security and Security Defense Systems Initiative, The Biodesign Institute, Arizona State University, 781 E. Terrace Mall, Tempe, AZ 85287-5904, USA.
David R ColquhounDepartment of Environmental Health Sciences, The Johns Hopkins University, Bloomberg School of Public Health, 615 N. Wolfe St., Baltimore, MD 21205, USA.
Kellogg J SchwabDepartment of Environmental Health Sciences, The Johns Hopkins University, Bloomberg School of Public Health, 615 N. Wolfe St., Baltimore, MD 21205, USA.
Rolf U HaldenCenter for Environmental Security and Security Defense Systems Initiative, The Biodesign Institute, Arizona State University, 781 E. Terrace Mall, Tempe, AZ 85287-5904, USA; Department of Environmental Health Sciences, The Johns Hopkins University, Bloomberg School of Public Health, 615 N. Wolfe St., Baltimore, MD 21205, USA. Electronic address: halden@asu.edu.
Johns Hopkins University · USArizona State University · US

Funding

Novel Approaches to Studying the Situ Bioremediation of Complex MixturesR01ES015445 · NIEHS · JOHNS HOPKINS UNIVERSITY · PI HALDEN, ROLF U · 2006 to 2009
$1.0M
In Situ Sampling Tool for Assessing Bioavailability and Toxicity of SedimentsR01ES020889 · NIEHS · ARIZONA STATE UNIVERSITY-TEMPE CAMPUS · PI DENSLOW, NANCY D, HALDEN, ROLF U · 2011 to 2013
$874k
NIEHS NIH HHS R01 ES015445NIEHS NIH HHS R01ES015445NIEHS NIH HHS R01 ES020889NIEHS NIH HHS R01ES020889
6 · The paper itself

Abstract

Norovirus infections are one of the most prominent public health problems of microbial origin in the U.S. and other industrialized countries. Surveillance is necessary to prevent secondary infection, confirm successful cleanup after outbreaks, and track the causative agent. Quantitative mass spectrometry, based on absolute quantitation with stable-isotope labeled peptides, is a promising tool for norovirus monitoring because of its speed, sensitivity, and robustness in the face of environmental inhibitors. In the current study, we present two new methods for the detection of the norovirus genogroup I capsid protein using electrospray and matrix-assisted laser desorption/ionization (MALDI) mass spectrometry. The peptide TLDPIEVPLEDVR was used to quantify norovirus-like particles down to 500 attomoles with electrospray and 100 attomoles with MALDI. With MALDI, we also demonstrate a detection limit of 1 femtomole and a quantitative dynamic range of 5 orders of magnitude in the presence of an environmental matrix effect. Due to the rapid processing time and applicability to a wide range of environmental sample types (bacterial lysate, produce, milk, soil, and groundwater), mass spectrometry-based absolute quantitation has a strong potential for use in public health and environmental sciences.

Indexed as

NorovirusAnimalsCapsid ProteinsFood MicrobiologyFragariaGroundwaterLactucaMilkPeptidesSoil MicrobiologySpectrometry, Mass, Electrospray IonizationSpectrometry, Mass, Matrix-Assisted Laser Desorption-IonizationWater MicrobiologyCapsid ProteinsPeptidesAbsolute quantificationEnvironmental detectionMass spectrometryNorovirus

Identifiers

PMID25603302
PMCPMC4369174
OpenAlexW2068603015

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.