ArticlePloS one2015
Regulated expression of a cytokinin biosynthesis gene IPT delays leaf senescence and improves yield under rainfed and irrigated conditions in canola (Brassica napus L.).
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers, 1 of them a synthesis that pooled it.
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Who cites it
41 citing papers in PubMed, 1 synthesis or guideline pooled it, 79 citations in OpenAlex.
- Role of cytokinins in seed development in pulses and oilseed crops: Current status and future perspective.Frontiers in genetics · 2022Pooled it
- From signals to solutions: stress-induced leaf senescence and synthetic biology and AI approaches for crop resilience.Molecular horticulture · 2026Review
- Evolutionary Repurposing of Cytokinin Signaling in Plant Development and Symbiosis.Plants (Basel, Switzerland) · 2026Review
- Differential activity of CYTOKININ OXIDASE/DEHYDROGENASE3 (CKX3) and CKX5 genes in regulating yield components in Brassica napus L.Plant molecular biology · 2026Article
- Biotechnological modification of the cytokinin regulatory system to improve drought and heat tolerance in the major crops.Plant cell reports · 2026Review
- Plant Signaling Hormones and Transcription Factors: Key Regulators of Plant Responses to Growth, Development, and Stress.Plants (Basel, Switzerland) · 2025Review
- Phytohormonal Regulation of Abiotic Stress Tolerance, Leaf Senescence and Yield Response in Field Crops: A Comprehensive Review.Biotech (Basel (Switzerland)) · 2025Review
- Metabolic flux analysis to increase oil in seeds.Plant physiology · 2025Review
- Nitric oxide and cytokinin cross-talk and their role in plant hypoxia response.Plant signaling & behavior · 2024Review
- Mechanisms of Cannabis Growth Promotion byPlants (Basel, Switzerland) · 2024Article
- Cytokinin: From autoclaved DNA to two-component signaling.The Plant cell · 2024Review
- Abiotic Stress-Induced Leaf Senescence: Regulatory Mechanisms and Application.International journal of molecular sciences · 2023Review
- Delaying or promoting? Manipulation of leaf senescence to improve crop yield and quality.Planta · 2023Review
- Optimized border irrigation delays winter wheat flag leaf senescence and promotes grain filling.Frontiers in plant science · 2023Article
- Modifications of Phytohormone Metabolism Aimed at Stimulation of Plant Growth, Improving Their Productivity and Tolerance to Abiotic and Biotic Stress Factors.Plants (Basel, Switzerland) · 2022Review
- The Relationships among "STAY-GREEN" Trait, Post-Anthesis Assimilate Remobilization, and Grain Yield in Rice (International journal of molecular sciences · 2022Article
- Cytokinin and abiotic stress tolerance -What has been accomplished and the way forward?Frontiers in genetics · 2022Review
- Overexpression ofFrontiers in plant science · 2022Article
- Stable and reproducible expression of bacterialFrontiers in plant science · 2022Article
- Research progress on the relationship between leaf senescence and quality, yield and stress resistance in horticultural plants.Frontiers in plant science · 2022Review
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Delay of leaf senescence through genetic modification can potentially improve crop yield, through maintenance of photosynthetically active leaves for a longer period. Plant growth hormones such as cytokinin regulate and delay leaf senescence. Here, the structural gene (IPT) encoding the cytokinin biosynthetic enzyme isopentenyltransferase was fused to a functionally active fragment of the AtMYB32 promoter and was transformed into canola plants. Expression of the AtMYB32xs::IPT gene cassette delayed the leaf senescence in transgenic plants grown under controlled environment conditions and field experiments conducted for a single season at two geographic locations. The transgenic canola plants retained higher chlorophyll levels for an extended period and produced significantly higher seed yield with similar growth and phenology compared to wild type and null control plants under rainfed and irrigated treatments. The yield increase in transgenic plants was in the range of 16% to 23% and 7% to 16% under rainfed and irrigated conditions, respectively, compared to control plants. Most of the seed quality parameters in transgenic plants were similar, and with elevated oleic acid content in all transgenic lines and higher oil content and lower glucosinolate content in one specific transgenic line as compared to control plants. The results suggest that by delaying leaf senescence using the AtMYB32xs::IPT technology, productivity in crop plants can be improved under water stress and well-watered conditions.
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