ArticlePloS one2015
Acute cutaneous wounds treated with human decellularised dermis show enhanced angiogenesis during healing.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 45 citations in OpenAlex.
- Advances in preparation of acellular human dermis for tissue banking and transplantation.Cell and tissue banking · 2024Article
- Surgical Treatment of Insertional Achilles Tendinopathy Augmented With Human Acellular Dermal Matrix: A Retrospective Case Series.Foot & ankle orthopaedics · 2024Article
- Decellularized Human Dermis for Orthoplastic Extremity Reconstruction.Bioengineering (Basel, Switzerland) · 2024Review
- Autologous Skin Grafts, versus Tissue-engineered Skin Constructs: A Systematic Review and Meta-analysis.Plastic and reconstructive surgery. Global open · 2023Article
- Acellular Dermal Matrix in Plastic and Reconstructive Surgery.Physiological research · 2022Article
- 3D-printed dermis-specific extracellular matrix mitigates scar contraction via inducing early angiogenesis and macrophage M2 polarization.Bioactive materials · 2022Article
- Classification of Distinct Endotypes in Human Skin Scarring: S.C.A.R.-A Novel Perspective on Dermal Fibrosis.Advances in wound care · 2022Article
- Exosomes derived from M2 macrophages induce angiogenesis to promote wound healing.Frontiers in molecular biosciences · 2022Article
- Further structural characterization of ovine forestomach matrix and multi-layered extracellular matrix composites for soft tissue repair.Journal of biomaterials applications · 2022Article
- Article
- Functional Testing of a Skin Topical FormulationAdvances in wound care · 2019Article
- Effect of Hypoxia on Gene Expression in Cell Populations Involved in Wound Healing.BioMed research international · 2019Article
- The effects of irradiation on the biological and biomechanical properties of an acellular porcine superflexor tendon graft for cruciate ligament repair.Journal of biomedical materials research. Part B, Applied biomaterials · 2017Article
- Retrospective observational analysis of the use of an architecturally unique dermal regeneration template (Derma Pure®) for the treatment of hard-to-heal wounds.International wound journal · 2017Article
- Significantly Accelerated Wound Healing of Full-Thickness Skin Using a Novel Composite Gel of Porcine Acellular Dermal Matrix and Human Peripheral Blood Cells.Cell transplantation · 2017Article
- Correction: Acute cutaneous wounds treated with human decellularised dermis show enhanced angiogenesis during healing.PloS one · 2015Article
- Augmentation of the insufficient tissue bed for surgical repair of hypospadias using acellular matrix grafts: A proof of concept study.Journal of tissue engineeringArticle
- Development and characterisation of a low-concentration sodium dodecyl sulphate decellularised porcine dermis.Journal of tissue engineeringArticle
Corrections and comments
- Erratum issued
Authors and funding
7 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe influence of skin substitutes upon angiogenesis during wound healing is unclear.
objectivesTo compare the angiogenic response in acute cutaneous human wounds treated with autogenic, allogenic and xenogenic skin substitutes to those left to heal by secondary intention.
methodsOn day 0, four 5mm full-thickness punch biopsies were harvested from fifty healthy volunteers (sites 1-4). In all cases, site 1 healed by secondary intention (control), site 2 was treated with collagen-GAG scaffold (CG), cadaveric decellularised dermis (DCD) was applied to site 3, whilst excised tissue was re-inserted into site 4 (autograft). Depending on study group allocation, healing tissue from sites 1-4 was excised on day 7, 14, 21 or 28. All specimens were bisected, with half used in histological and immunohistochemical evaluation whilst extracted RNA from the remainder enabled whole genome microarrays and qRT-PCR of highlighted angiogenesis-related genes. All wounds were serially imaged over 6 weeks using laser-doppler imaging and spectrophotometric intracutaneous analysis.
resultsInherent structural differences between skin substitutes influenced the distribution and organisation of capillary networks within regenerating dermis. Haemoglobin flux (p = 0.0035), oxyhaemoglobin concentration (p = 0.0005), and vessel number derived from CD31-based immunohistochemistry (p = 0.046) were significantly greater in DCD wounds at later time points. This correlated with time-matched increases in mRNA expression of membrane-type 6 matrix metalloproteinase (MT6-MMP) (p = 0.021) and prokineticin 2 (PROK2) (p = 0.004).
conclusionCorroborating evidence from invasive and non-invasive modalities demonstrated that treatment with DCD resulted in increased angiogenesis after wounding. Significantly elevated mRNA expression of pro-angiogenic PROK2 and extracellular matrix protease MT6-MMP seen only in the DCD group may contribute to observed responses.
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