ArticleThe Plant journal : for cell and molecular biology2015
A tonoplast Glu/Asp/GABA exchanger that affects tomato fruit amino acid composition.
Article in The Plant journal : for cell and molecular biology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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Who cites it
46 citing papers in PubMed, 95 citations in OpenAlex.
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- Comparative transcriptome analysis revealed that tomato response to salt stress induced by exogenous GABA and functional verification of SlGAD1.BMC plant biology · 2025Article
- Dynamic Characterization of Antioxidant-Related, Non-Volatile, and Volatile Metabolite Profiles of Cherry Tomato During Ripening.Antioxidants (Basel, Switzerland) · 2025Article
- JA-Mediated Regulation of Amino Acid Homeostasis Adjusts Metabolic Flux and Enhances Spider Mite Tolerance via the SlJAZ8-SlWRKY57-SlAVT6s Module in Tomato.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Phytochemical and metabolic changes associated with ripening of Lycopersicon esculentum.Scientific reports · 2025Article
- The amino acid permeaseHorticulture research · 2025Article
- Advances in Plant GABA Research: Biological Functions, Synthesis Mechanisms and Regulatory Pathways.Plants (Basel, Switzerland) · 2024Review
- Gamma-aminobutyric acid interactions with phytohormones and its role in modulating abiotic and biotic stress in plants.Stress biology · 2024Review
- ComparativeFoods (Basel, Switzerland) · 2024Article
- Types of Membrane Transporters and the Mechanisms of Interaction between Them and Reactive Oxygen Species in Plants.Antioxidants (Basel, Switzerland) · 2024Review
- Recent Advances in Studying the Regulation of Fruit Ripening in Tomato Using Genetic Engineering Approaches.International journal of molecular sciences · 2024Review
- Enhancement of specialized metabolites using CRISPR/Cas gene editing technology in medicinal plants.Frontiers in plant science · 2024Review
- Response of soybean root exudates and related metabolic pathways to low phosphorus stress.PloS one · 2024Article
- Why did glutamate, GABA, and melatonin become intercellular signalling molecules in plants?eLife · 2023Review
- GABA Metabolism, Transport and Their Roles and Mechanisms in the Regulation of Abiotic Stress (Hypoxia, Salt, Drought) Resistance in Plants.Metabolites · 2023Review
- Effect of Light Quality on Metabolomic, Ionomic, and Transcriptomic Profiles in Tomato Fruit.International journal of molecular sciences · 2022Article
- The Tiny Companion Matters: The Important Role of Protons in Active Transports in Plants.International journal of molecular sciences · 2022Review
- Article
- γ-Aminobutyrate Improves the Postharvest Marketability of Horticultural Commodities: Advances and Prospects.Frontiers in plant science · 2022Review
- Defective cytokinin signaling reprograms lipid and flavonoid gene-to-metabolite networks to mitigate high salinity inProceedings of the National Academy of Sciences of the United States of America · 2021Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Vacuolar accumulation of acidic metabolites is an important aspect of tomato fruit flavour and nutritional quality. The amino acids Asp and Glu accumulate to high concentrations during ripening, while γ-aminobutyrate (GABA) shows an approximately stoichiometric decline. Given that GABA can be catabolised to form Glu and subsequently Asp, and the requirement for the fruit to maintain osmotic homeostasis during ripening, we hypothesised the existence of a tonoplast transporter that exports GABA from the vacuole in exchange for import of either Asp or Glu. We show here that the tomato vacuolar membrane possesses such a transport property: transport of Glu across isolated tonoplast vesicle membranes was trans-stimulated in counterexchange mode by GABA, Glu and Asp. We identified SlCAT9 as a candidate protein for this exchanger using quantitative proteomics of a tonoplast-enriched membrane fraction. Transient expression of a SlCAT9-YFP fusion in tobacco confirmed a tonoplast localisation. The function of the protein was examined by overexpression of SlCAT9 in transgenic tomato plants. Tonoplast vesicles isolated from transgenic plants showed higher rates of Glu and GABA transport than wild-type (WT) only when assayed in counterexchange mode with Glu, Asp, or GABA. Moreover, there were substantial increases in the content of all three cognate amino acids in ripe fruit from the transgenic plants. We conclude that SlCAT9 is a tonoplast Glu/Asp/GABA exchanger that strongly influences the accumulation of these amino acids during fruit development.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.