ReviewFrontiers in plant science2014
Positive-negative-selection-mediated gene targeting in rice.
Review in Frontiers in plant science, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
27 citing papers in PubMed, 46 citations in OpenAlex.
- Review
- Gene Targeting via CRISPR/Cas9-Mediated DNA Double-Strand Break Induction in Rice.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Precise genetic engineering withPlant biotechnology (Tokyo, Japan) · 2023Article
- CRISPR/Cas9-mediated seamless gene replacement in protoplasts expands the resistance spectrum to TMV-U1 strain in regenerated Nicotiana tabacum.Plant biotechnology journal · 2023Article
- Recent Advances in Antibiotic-Free Markers; Novel Technologies to Enhance Safe Human Food Production in the World.Molecular biotechnology · 2023Review
- Genome Editing Technology and Its Application to Metabolic Engineering in Rice.Rice (New York, N.Y.) · 2022Review
- Gene Targeting Facilitated by Engineered Sequence-Specific Nucleases: Potential Applications for Crop Improvement.Plant & cell physiology · 2021Review
- Sequence modification on demand: search and replace tools for precise gene editing in plants.Transgenic research · 2021Review
- Novel assays to monitor gene expression and protein-protein interactions in rice using the bioluminescent protein, NanoLuc.Plant biotechnology (Tokyo, Japan) · 2021Article
- Precision genome editing in plants via gene targeting and subsequent break-induced single-strand annealing.Plant biotechnology journal · 2021Article
- Enhanced NRT1.1/NPF6.3 expression in shoots improves growth under nitrogen deficiency stress in Arabidopsis.Communications biology · 2021Article
- Characterization of a new rice OsMADS1 null mutant generated by homologous recombination-mediated gene targeting.Planta · 2021Article
- Rice Gene Targeting by Homologous Recombination with Positive-Negative Selection Strategy.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Real-Time Monitoring of Key Gene Products Involved in Rice Photoperiodic Flowering.Frontiers in plant science · 2021Article
- Precise Genome Editing in miRNA Target Site via Gene Targeting and Subsequent Single-Strand-Annealing-Mediated Excision of the Marker Gene in Plants.Frontiers in genome editing · 2020Article
- Article
- Data Mining by Pluralistic Approach on CRISPR Gene Editing in Plants.Frontiers in plant science · 2019Review
- CRISPR/Cas9-mediated gene targeting in Arabidopsis using sequential transformation.Nature communications · 2018Article
- The evolution of CRISPR/Cas9 and their cousins: hope or hype?Biotechnology letters · 2018Review
- Homology-based double-strand break-induced genome engineering in plants.Plant cell reports · 2016Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Gene targeting (GT) refers to the designed modification of genomic sequence(s) through homologous recombination (HR). GT is a powerful tool both for the study of gene function and for molecular breeding. However, in transformation of higher plants, non-homologous end joining (NHEJ) occurs overwhelmingly in somatic cells, masking HR-mediated GT. Positive-negative selection (PNS) is an approach for finding HR-mediated GT events because it can eliminate NHEJ effectively by expression of a negative-selection marker gene. In rice-a major crop worldwide-reproducible PNS-mediated GT of endogenous genes has now been successfully achieved. The procedure is based on strong PNS using diphtheria toxin A-fragment as a negative marker, and has succeeded in the directed modification of several endogenous rice genes in various ways. In addition to gene knock-outs and knock-ins, a nucleotide substitution in a target gene was also achieved recently. This review presents a summary of the development of the rice PNS system, highlighting its advantages. Different types of gene modification and gene editing aimed at developing new plant breeding technology based on PNS are discussed.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.