ArticleThe Journal of cell biology2015
Multiple assembly mechanisms anchor the KMN spindle checkpoint platform at human mitotic kinetochores.
Article in The Journal of cell biology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 88 papers.
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Who cites it
88 citing papers in PubMed, 138 citations in OpenAlex.
- Structure and function of the outer kinetochore.Nature reviews. Molecular cell biology · 2026Review
- α-Satellite RNA maintains centromeric cohesion through R-loops.Molecular cell · 2026Article
- A NEK2/ZWINT-NDC80 regulatory axis drives bladder cancer progression and chemoresistance.Discover oncology · 2026Article
- Assembly and phosphoregulatory mechanisms of the budding yeast outer kinetochore KMN complex.The Journal of cell biology · 2026Article
- Microtubule end stabilisation by cooperative oligomers of Ska and Ndc80 complexes.The EMBO journal · 2026Article
- KIF18A promotes chromosome congression in cooperation with CENP-E downstream of CENP-C.Cell reports · 2025Article
- CENcyclopedia: dynamic landscape of kinetochore architecture throughout the cell cycle.Nature communications · 2025Article
- Interplay of kinetochores and catalysts drives rapid assembly of the mitotic checkpoint complex.Nature communications · 2025Article
- The mFrontiers in oncology · 2025Article
- CENP-C-Mis12 complex establishes a regulatory loop through Aurora B for chromosome segregation.Life science alliance · 2025Article
- Dynamics of spindle assembly and position checkpoints: Integrating molecular mechanisms with computational models.Computational and structural biotechnology journal · 2025Review
- Binding site maturation modulated by molecular density underlies Ndc80 binding to kinetochore receptor CENP-T.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Molecular details and phosphoregulation of the CENP-T-Mis12 complex interaction during mitosis in DT40 cells.iScience · 2024Article
- A conserved germline-specific Dsn1 alternative splice isoform supports oocyte and embryo development.Current biology : CB · 2024Article
- Structure of the human KMN complex and implications for regulation of its assembly.Nature structural & molecular biology · 2024Article
- Structure of the human outer kinetochore KMN network complex.Nature structural & molecular biology · 2024Article
- Topoisomerase I is an Evolutionarily Conserved Key Regulator for Satellite DNA Transcription.bioRxiv : the preprint server for biology · 2024Article
- Coregulation of NDC80 Complex Subunits Determines the Fidelity of the Spindle-Assembly Checkpoint and Mitosis.Molecular cancer research : MCR · 2024Article
- A conserved germline-specific Dsn1 alternative splice isoform supports oocyte and embryo development.bioRxiv : the preprint server for biology · 2024Article
- Higher-order protein assembly controls kinetochore formation.Nature cell biology · 2024Article
28 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
Abstract
During mitosis, the spindle checkpoint senses kinetochores not properly attached to spindle microtubules and prevents precocious sister-chromatid separation and aneuploidy. The constitutive centromere-associated network (CCAN) at inner kinetochores anchors the KMN network consisting of Knl1, the Mis12 complex (Mis12C), and the Ndc80 complex (Ndc80C) at outer kinetochores. KMN is a critical kinetochore receptor for both microtubules and checkpoint proteins. Here, we show that nearly complete inactivation of KMN in human cells through multiple strategies produced strong checkpoint defects even when all kinetochores lacked microtubule attachment. These KMN-inactivating strategies reveal multiple KMN assembly mechanisms at human mitotic kinetochores. In one mechanism, the centromeric kinase Aurora B phosphorylates Mis12C and strengthens its binding to the CCAN subunit CENP-C. In another, CENP-T contributes to KMN attachment in a CENP-H-I-K-dependent manner. Our study provides insights into the mechanisms of mitosis-specific assembly of the checkpoint platform KMN at human kinetochores.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.