ArticleClinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases2015
How well does physician selection of microbiologic tests identify Clostridium difficile and other pathogens in paediatric diarrhoea? Insights using multiplex PCR-based detection.
Article in Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers, 1 of them a synthesis that pooled it.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
25 citing papers in PubMed, 1 synthesis or guideline pooled it, 60 citations in OpenAlex.
- Systematic Review on the Correlation of Quantitative PCR Cycle Threshold Values of Gastrointestinal Pathogens With Patient Clinical Presentation and Outcomes.Frontiers in medicine · 2021Pooled it
- Mapping the Literature on the Impact of Gastrointestinal Multiplexed Pathogen Panels on Clinical and Healthcare Utilization Outcomes: A Scoping Review.The journal of applied laboratory medicine · 2026Article
- Patterns of gastrointestinal pathogen co-detection in pediatric stool samples identified by rapid multiplex PCR.Epidemiology and infection · 2026Article
- PCR-Based Versus Conventional Stool Testing in Hospitalized Patients with Diarrhea: Diagnostic Yield, Clinical Impact, and Stewardship Implications.Microorganisms · 2025Article
- Use of PCR cycle threshold values for toxins A and B quantification in Clostridioides difficile infections.Scientific reports · 2025Article
- Risk factors and clinical outcomes associated with multiple as opposed to single pathogens detected on the gastrointestinal disease polymerase chain reaction assay.Gut pathogens · 2024Article
- Article
- Conventional and advanced detection techniques of foodborne pathogens: A comprehensive review.Heliyon · 2023Article
- Comparative Evaluation of Luminex xTAGMicroorganisms · 2022Article
- Real-time gastrointestinal infection surveillance through a cloud-based network of clinical laboratories.PloS one · 2021Article
- Multi-organism gastrointestinal polymerase chain reaction positivity among pediatric transplant vs non-transplant populations: A single-center experience.Pediatric transplantation · 2020Article
- Do changes in STEC diagnostics mislead interpretation of disease surveillance data in Switzerland? Time trends in positivity, 2007 to 2016.Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin · 2020Article
- Use of BioFire FilmArray gastrointestinal PCR panel associated with reductions in antibiotic use, time to optimal antibiotics, and length of stay.BMC gastroenterology · 2020Article
- Evaluation of viral co-infections among patients with community-associated Clostridioides difficile infection.PloS one · 2020Article
- Impact of the BioFire FilmArray gastrointestinal panel on patient care and infection control.PloS one · 2020Article
- Molecular Diagnostic Advances in Transplant Infectious Diseases.Current infectious disease reports · 2019Review
- New Developments in Rapid Diagnostic Testing for Children.Infectious disease clinics of North America · 2018Review
- Advances in the diagnosis and treatment of Clostridium difficile infections.Emerging microbes & infections · 2018Review
- Fecal Host Transcriptomics for Non-Invasive Human Mucosal Immune Profiling: Proof of Concept inPathogens & immunity · 2018Article
- Article
Corrections and comments
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Authors and funding
11 authors at 3 institutions in 1 country.
Funding
Abstract
The objective of this study was to compare the aetiologic yield of standard-of-care microbiologic testing ordered by physicians with that of a multiplex PCR platform. Stool specimens obtained from children and young adults with gastrointestinal illness were evaluated by standard laboratory methods and a developmental version of the FilmArray Gastrointestinal (GI) Diagnostic System (FilmArray GI Panel), a rapid multiplex PCR platform that detects 23 bacterial, viral and protozoal agents. Results were classified according to the microbiologic tests requested by the treating physician. A median of three (range 1-10) microbiologic tests were performed by the clinical laboratory during 378 unique diarrhoeal episodes. A potential aetiologic agent was identified in 46% of stool specimens by standard laboratory methods and in 65% of specimens tested using the FilmArray GI Panel (p < 0.001). For those patients who only had Clostridium difficile testing requested, an alternative pathogen was identified in 29% of cases with the FilmArray GI Panel. Notably, 11 (12%) cases of norovirus were identified among children who only had testing for Clostridium difficile ordered. Among those who had C. difficile testing ordered in combination with other tests, an additional pathogen was identified in 57% of stool specimens with the FilmArray GI Panel. For patients who had no C. difficile testing performed, the FilmArray GI Panel identified a pathogen in 63% of cases, including C. difficile in 8%. Physician-specified laboratory testing may miss important diarrhoeal pathogens. Additionally, standard laboratory testing is likely to underestimate co-infections with multiple infectious diarrhoeagenic agents.
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Registered trials
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