ArticleThe New phytologist2015
ZmpTAC12 binds single-stranded nucleic acids and is essential for accumulation of the plastid-encoded polymerase complex in maize.
Article in The New phytologist, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 31 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
31 citing papers in PubMed, 58 citations in OpenAlex.
- OsFeSOD3 Functions as an Enzymatic Component of the PEP Complex, Bifunctionally Regulating Chloroplastic ROS Metabolism and Chloroplast Biogenesis in Rice.Plant biotechnology journal · 2026Article
- A red/blue optoswitch for temporal control of chloroplast transcription and biogenesis in Arabidopsis.Nature communications · 2026Article
- The PSRP2/4 Proteins Promote Viral Infection by Interacting with the VPg Protein of TuMV.Plants (Basel, Switzerland) · 2025Article
- Regreening mechanisms in cucumber: insights from a CsSIG2 mutation affecting chloroplast development.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2025Article
- Comparative analyses of chloroplast genomes inFrontiers in plant science · 2025Article
- Architecture of the spinach plastid-encoded RNA polymerase.Nature communications · 2024Article
- Regulation of chloroplast biogenesis, development, and signaling by endogenous and exogenous cues.Physiology and molecular biology of plants : an international journal of functional plant biology · 2024Review
- Chloroplast gene expression: Recent advances and perspectives.Plant communications · 2023Review
- Plant photoreceptors and their signalling components in chloroplastic anterograde and retrograde communication.Journal of experimental botany · 2022Article
- High-resolution map of plastid-encoded RNA polymerase binding patterns demonstrates a major role of transcription in chloroplast gene expression.The Plant journal : for cell and molecular biology · 2022Article
- Retrograde and anterograde signaling in the crosstalk between chloroplast and nucleus.Frontiers in plant science · 2022Review
- Plant height heterosis is quantitatively associated with expression levels of plastid ribosomal proteins.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- PPR647 Protein Is Required for Chloroplast RNA Editing, Splicing and Chloroplast Development in Maize.International journal of molecular sciences · 2021Article
- Nucleo-plastidic PAP8/pTAC6 couples chloroplast formation with photomorphogenesis.The EMBO journal · 2020Article
- Genome communication in plants mediated by organelle-n-ucleus-located proteins.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2020Review
- The plastid transcription machinery and its coordination with the expression of nuclear genome: Plastid-Encoded Polymerase, Nuclear-Encoded Polymerase and the Genomes Uncoupled 1-mediated retrograde communication.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2020Review
- Article
- mTERF8, a Member of the Mitochondrial Transcription Termination Factor Family, Is Involved in the Transcription Termination of Chloroplast GenePlant physiology · 2020Article
- An isoform of the plastid RNA polymerase-associated protein FSD3 negatively regulates chloroplast development.BMC plant biology · 2019Article
- PPR-SMR1 is required for the splicing of multiple mitochondrial introns, interacts with Zm-mCSF1, and is essential for seed development in maize.Journal of experimental botany · 2019Article
Corrections and comments
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- Erratum issued
Authors and funding
6 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The plastid-encoded plastid RNA polymerase (PEP) represents the major transcription machinery in mature chloroplasts. Proteomic studies identified four plastome- and at least ten nuclear-encoded proteins making up this multimeric enzyme. Depletion of single subunits is known to result in strongly diminished PEP activity causing severe defects in chloroplast biogenesis. Here, we characterized one PEP subunit in maize, ZmpTAC12, and investigated the molecular basis underlying PEP-deficiency in Zmptac12 mutants. We show that the ZmpTAC12 gene encodes two different protein isoforms, both of which localize dually in chloroplasts and nuclei. Moreover, both variants assemble into the PEP-complex. Analysis of PEP-complex assembly in various maize mutants lacking different PEP-complex components demonstrates that ZmpTAC12, ZmpTAC2, ZmpTAC10 and ZmMurE are each required to accumulate a fully assembled PEP-complex. Antibodies to ZmpTAC12 coimmunoprecipitate a subset of plastid RNAs that are synthesized by PEP-dependent transcription. Gel mobility shift analyses with recombinant ZmpTAC12 revealed binding capabilities with ssRNA and ssDNA, but not dsDNA. Collectively these data demonstrate that ZmpTAC12 is required for the proper build-up of the PEP-complex and that it interacts with single-stranded nucleic acids.
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