ArticleStructure (London, England : 1993)2015
Structural and evolutionary analyses show unique stabilization strategies in the type IV pili of Clostridium difficile.
Article in Structure (London, England : 1993), 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 49 citations in OpenAlex.
- Insight into the relationship between type IV pilus function and biofilm formation.Biochemical Society transactions · 2026Review
- Dissecting the role of flagellar subunits inJournal of bacteriology · 2025Article
- Clostridioides difficile Biofilm.Advances in experimental medicine and biology · 2024Article
- Landmark Discoveries and Recent Advances in Type IV Pilus Research.Microbiology and molecular biology reviews : MMBR · 2022Review
- The Type IV Pilus of Plasmid TP114 Displays Adhesins Conferring Conjugation Specificity and Is Important for DNA Transfer in the Mouse Gut Microbiota.Microbiology spectrum · 2022Article
- Engineering Natural Competence into the Fast-Growing CyanobacteriumApplied and environmental microbiology · 2022Article
- The Role of Minor Pilins in Assembly and Function of the Competence Pilus ofFrontiers in cellular and infection microbiology · 2021Article
- Caldicellulosiruptor bescii Adheres to Polysaccharides via a Type IV Pilin-Dependent Mechanism.Applied and environmental microbiology · 2020Article
- A practical overview of molecular replacement: Clostridioides difficile PilA1, a difficult case study.Acta crystallographica. Section D, Structural biology · 2020Article
- Monoderm bacteria: the new frontier for type IV pilus biology.Molecular microbiology · 2019Review
- Protein Nanotubes: From Bionanotech towards Medical Applications.Biomedicines · 2019Review
- Global biochemical and structural analysis of the type IV pilus from the Gram-positive bacteriumThe Journal of biological chemistry · 2019Article
- The Dynamic Structures of the Type IV Pilus.Microbiology spectrum · 2019Review
- The structure of PilA fromThe Journal of biological chemistry · 2019Article
- Gram-Positive Type IV Pili and Competence.Microbiology spectrum · 2019Article
- DNA Uptake by Type IV Filaments.Frontiers in molecular biosciences · 2019Review
- Comparative inhibitory profile and distribution of bacterial PARPs, using Clostridioides difficile CD160 PARP as a model.Scientific reports · 2018Article
- Type IV Pili Promote Clostridium difficile Adherence and Persistence in a Mouse Model of Infection.Infection and immunity · 2018Article
- TargetingFrontiers in microbiology · 2018Review
- Surfaceome and Proteosurfaceome in Parietal Monoderm Bacteria: Focus on Protein Cell-Surface Display.Frontiers in microbiology · 2018Review
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Authors and funding
10 authors at 2 institutions in 2 countries.
Funding
Abstract
Type IV pili are produced by many pathogenic Gram-negative bacteria and are important for processes as diverse as twitching motility, biofilm formation, cellular adhesion, and horizontal gene transfer. However, many Gram-positive species, including Clostridium difficile, also produce type IV pili. Here, we identify the major subunit of the type IV pili of C. difficile, PilA1, and describe multiple 3D structures of PilA1, demonstrating the diversity found in three strains of C. difficile. We also model the incorporation of both PilA1 and a minor pilin, PilJ, into the pilus fiber. Although PilA1 contains no cysteine residues, and therefore cannot form the disulfide bonds found in all Gram-negative type IV pilins, it adopts unique strategies to achieve a typical pilin fold. The structures of PilA1 and PilJ exhibit similarities with the type IVb pilins from Gram-negative bacteria that suggest that the type IV pili of C. difficile are involved in microcolony formation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.