ArticleeLife2015
Methylation at the C-2 position of hopanoids increases rigidity in native bacterial membranes.
Article in eLife, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- Methanotroph-methylotroph lipid adaptations to changing environmental conditions.Frontiers in microbiology · 2025Article
- Hopanoid lipids promote soybeanmBio · 2024Article
- Bacterial lipid biophysics and membrane organization.Current opinion in microbiology · 2023Review
- Integrative Genomics Sheds Light on Evolutionary Forces Shaping the Acidithiobacillia Class Acidophilic Lifestyle.Frontiers in microbiology · 2021Article
- Vitamin BProceedings of the National Academy of Sciences of the United States of America · 2020Article
- A High-Efficacy CRISPR Interference System for Gene Function Discovery in Zymomonas mobilis.Applied and environmental microbiology · 2020Article
- Role of the lipid bilayer in outer membrane protein folding in Gram-negative bacteria.The Journal of biological chemistry · 2020Review
- Extended Hopanoid Loss Reduces Bacterial Motility and Surface Attachment and Leads to Heterogeneity in Root Nodule Growth Kinetics in aMolecular plant-microbe interactions : MPMI · 2019Article
- Hopanoid lipids: from membranes to plant-bacteria interactions.Nature reviews. Microbiology · 2018Review
- Lack of Methylated Hopanoids Renders the Cyanobacterium Nostoc punctiforme Sensitive to Osmotic and pH Stress.Applied and environmental microbiology · 2017Article
- Hopanoid-free Methylobacterium extorquens DM4 overproduces carotenoids and has widespread growth impairment.PloS one · 2017Article
- Article
- Hopanoids as functional analogues of cholesterol in bacterial membranes.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
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Authors and funding
3 authors.
Funding
Abstract
Sedimentary rocks host a vast reservoir of organic carbon, such as 2-methylhopane biomarkers, whose evolutionary significance we poorly understand. Our ability to interpret this molecular fossil record is constrained by ignorance of the function of their molecular antecedents. To gain insight into the meaning of 2-methylhopanes, we quantified the dominant (des)methylated hopanoid species in the membranes of the model hopanoid-producing bacterium Rhodopseudomonas palustris TIE-1. Fluorescence polarization studies of small unilamellar vesicles revealed that hopanoid 2-methylation specifically renders native bacterial membranes more rigid at concentrations that are relevant in vivo. That hopanoids differentially modify native membrane rigidity as a function of their methylation state indicates that methylation itself promotes fitness under stress. Moreover, knowing the in vivo (2Me)-hopanoid concentration range in different cell membranes, and appreciating that (2Me)-hopanoids' biophysical effects are tuned by the lipid environment, permits the design of more relevant in vitro experiments to study their physiological functions.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.