ArticleThe Analyst2015
Online SERS detection and characterization of eight biologically-active peptides separated by capillary zone electrophoresis.
Article in The Analyst, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
12 citing papers in PubMed, 28 citations in OpenAlex.
- Digital surface enhanced Raman spectroscopy for quantifiable single molecule detection in flow.The Analyst · 2024Article
- Raman and Surface-Enhanced Raman Scattering Detection in Flowing Solutions for Complex Mixture Analysis.Annual review of analytical chemistry (Palo Alto, Calif.) · 2024Review
- A 3D printed sheath flow interface for surface enhanced Raman spectroscopy (SERS) detection in flow.The Analyst · 2024Article
- SERS Investigation on Oligopeptides Used as Biomimetic Coatings for Medical Devices.Biomolecules · 2021Article
- Microchip isotachophoresis coupled to surface-enhanced Raman spectroscopy for pharmaceutical analysis.Mikrochimica acta · 2020Article
- Untargeted Tumor Metabolomics with Liquid Chromatography-Surface-Enhanced Raman Spectroscopy.Angewandte Chemie (International ed. in English) · 2020Article
- Application of Capillary Electrophoresis with Laser-Induced Fluorescence to Immunoassays and Enzyme Assays.Molecules (Basel, Switzerland) · 2019Review
- Online Liquid Chromatography-Sheath-Flow Surface Enhanced Raman Detection of Phosphorylated Carbohydrates.Analytical chemistry · 2018Article
- Bioanalytical applications of surface-enhanced Raman spectroscopy:Reviews in analytical chemistry · 2017Article
- Sheath flow SERS for chemical profiling in urine.Faraday discussions · 2016Article
- Increased SERS detection efficiency for characterizing rare events in flow.Analytical chemistry · 2015Article
- Sheath-flow microfluidic approach for combined surface enhanced Raman scattering and electrochemical detection.Analytical chemistry · 2015Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
There is a need for low cost, sensitive and chemical specific detectors for routine characterization of biomolecules. In this study, we utilize sheath-flow surface-enhanced Raman scattering (SERS) to analyze a mixture of eight biologically-active peptides separated by capillary zone electrophoresis (CZE). Analysis of the SERS electropherogram resulting from online detection resolves the characteristic Raman bands attributed to the amino acid constituents of each peptide, which enables identification. The detection limit by SERS was found to be 10(-8) M. Our results suggest that the structural information obtained from the detected vibrational modes provides complementary characterization to other chemically specific detectors like mass spectrometry and improved chemical identification over other commonly used optical-based post-chromatographic detection methods. In addition, the sheath-flow SERS detection results in band narrowing in the observed electropherogram that enables distinction of closely migrating species. The results presented here indicate that this platform can provide fast, robust, reproducible, and chemical specific detection to facilitate the characterization of peptides.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.