ArticleMobile DNA2014
A droplet digital PCR detection method for rare L1 insertions in tumors.
Article in Mobile DNA, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 20 citations in OpenAlex.
- The glioblastoma virome across tumor and extracellular compartments.Frontiers in oncology · 2025Article
- Article
- Review
- Subfamily-specific quantification of endogenous mouse L1 retrotransposons by droplet digital PCR.Analytical biochemistry · 2020Article
- Review
- Ultrasensitive Detection of PbJournal of analytical methods in chemistry · 2019Article
- Analysis of LINE-1 Elements in DNA from Postmortem Brains of Individuals with Schizophrenia.Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology · 2017Article
- Reading LINEs within the cocaine addicted brain.Brain and behavior · 2017Article
- L1-associated genomic regions are deleted in somatic cells of the healthy human brain.Nature neuroscience · 2016Article
- Optimized Detection of Plasmodium falciparum Topoisomerase I Enzyme Activity in a Complex Biological Sample by the Use of Molecular Beacons.Sensors (Basel, Switzerland) · 2016Article
- Deciphering fact from artifact when using reporter assays to investigate the roles of host factors on L1 retrotransposition.Mobile DNA · 2016Article
- Restricting retrotransposons: a review.Mobile DNA · 2016Review
- Response of transposable elements to environmental stressors.Mutation research. Reviews in mutation researchReview
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundThe active human mobile element, long interspersed element 1 (L1) currently populates human genomes in excess of 500,000 copies per haploid genome. Through its mobility via a process called target primed reverse transcription (TPRT), L1 mobilization has resulted in over 100 de novo cases of human disease and has recently been associated with various cancer types. Large advances in high-throughput sequencing (HTS) technology have allowed for an increased understanding of the role of L1 in human cancer; however, researchers are still limited by the ability to validate potentially rare L1 insertion events detected by HTS that may occur in only a small fraction of tumor cells. Additionally, HTS detection of rare events varies greatly as a function of read depth, and new tools for de novo element discovery are needed to fill in gaps created by HTS.
resultsWe have employed droplet digital PCR (ddPCR) to detect rare L1 loci in mosaic human genomes. Our assay allows for the detection of L1 insertions as rare as one cell in every 10,000.
conclusionsddPCR represents a robust method to be used alongside HTS techniques for detecting, validating and quantitating rare L1 insertion events in tumors and other tissues.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.