ArticleScientific reports2015
The role of myosin-II in force generation of DRG filopodia and lamellipodia.
Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 37 citations in OpenAlex.
- Myosin II-independent contraction of actin filaments in membrane nanotubes.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
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- Non-muscle myosin II inhibition at the site of axon injury increases axon regeneration.Nature communications · 2025Article
- β-catenin-α-catenin and actomyosin signaling differentially regulate growth cone contours and axon undulation and branching of retinal ganglion cellsFrontiers in cellular neuroscience · 2025Article
- Topological Distribution of Wound Stiffness Modulates Wound-Induced Hair Follicle Neogenesis.Pharmaceutics · 2022Article
- Binding Networks Identify Targetable Protein Pockets for Mechanism-Based Drug Design.International journal of molecular sciences · 2022Article
- Herpes simplex virus-1 utilizes the host actin cytoskeleton for its release from axonal growth cones.PLoS pathogens · 2022Article
- Non-Muscle Myosin II in Axonal Cell Biology: From the Growth Cone to the Axon Initial Segment.Cells · 2020Review
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- Mechanics of Pollen Tube Elongation: A Perspective.Frontiers in plant science · 2020Review
- p90 ribosomal S6 kinase (RSK) phosphorylates myosin phosphatase and thereby controls edge dynamics during cell migration.The Journal of biological chemistry · 2019Article
- Remarkable structural transformations of actin bundles are driven by their initial polarity, motor activity, crosslinking, and filament treadmilling.PLoS computational biology · 2019Article
- Axon growth regulation by a bistable molecular switch.Proceedings. Biological sciences · 2018Article
- Selective Regulation of Cytoskeletal Dynamics and Filopodia Formation by Teleost Leukocyte Immune-Type Receptors Differentially Contributes to Target Capture During the Phagocytic Process.Frontiers in immunology · 2018Article
- Cell-cell adhesion interface: orthogonal and parallel forces from contraction, protrusion, and retraction.F1000Research · 2018Review
- Small Rho GTPases and the Effector VipA Mediate the Invasion of Epithelial Cells by FilamentousFrontiers in cellular and infection microbiology · 2018Article
- Cell migration, intercalation and growth regulate mammalian cochlear extension.Development (Cambridge, England) · 2017Article
- Analysis of microtubule growth dynamics arising from altered actin network structure and contractility in breast tumor cells.Physical biology · 2017Article
- Axon tension regulates fasciculation/defasciculation through the control of axon shaft zippering.eLife · 2017Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Differentiating neurons process the mechanical stimulus by exerting the protrusive forces through lamellipodia and filopodia. We used optical tweezers, video imaging and immunocytochemistry to analyze the role of non-muscle myosin-II on the protrusive force exerted by lamellipodia and filopodia from developing growth cones (GCs) of isolated Dorsal Root Ganglia (DRG) neurons. When the activity of myosin-II was inhibited by 30 μM Blebbistatin protrusion/retraction cycles of lamellipodia slowed down and during retraction lamellipodia could not lift up axially as in control condition. Inhibition of actin polymerization with 25 nM Cytochalasin-D and of microtubule polymerization with 500 nM Nocodazole slowed down the protrusion/retraction cycles, but only Cytochalasin-D decreased lamellipodia axial motion. The force exerted by lamellipodia treated with Blebbistatin decreased by 50%, but, surprisingly, the force exerted by filopodia increased by 20-50%. The concomitant disruption of microtubules caused by Nocodazole abolished the increase of the force exerted by filopodia treated with Blebbistatin. These results suggest that; i- Myosin-II controls the force exerted by lamellipodia and filopodia; ii- contractions of the actomyosin complex formed by filaments of actin and myosin have an active role in ruffle formation; iii- myosin-II is an essential component of the structural stability of GCs architecture.
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