ArticleMolecular plant pathology2015
The pep4 gene encoding proteinase A is involved in dimorphism and pathogenesis of Ustilago maydis.
Article in Molecular plant pathology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 33 citations in OpenAlex.
- Comparative Analysis of Lifecycle-Dependent A to I RNA Editing in Three Members of theJournal of fungi (Basel, Switzerland) · 2026Article
- Aspartic Proteases Drive Stress Response, Morphogenesis, and Macrophage Interaction in Sporothrix brasiliensis.Mycopathologia · 2025Article
- Retromer Regulates Macro- and Micro-Autophagy via Distinct Vacuolar Proteases in the Rice Blast Fungus.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Vacuolar Proteases ofJournal of fungi (Basel, Switzerland) · 2025Article
- Characterization of Aspartic Proteases fromJournal of fungi (Basel, Switzerland) · 2023Article
- Article
- Intracellular Aminopeptidase Activity Determination from the Fungus Sporisorium reilianum: Purification and Biochemical Characterization of psrAPEi Enzyme.Current microbiology · 2022Article
- Construction and analysis of gene co-expression network in the pathogenic fungusFrontiers in microbiology · 2022Article
- Pathogenic Process-Associated Transcriptome Analysis ofInternational journal of genomics · 2022Article
- Vacuolar proteases and autophagy in phytopathogenic fungi: A review.Frontiers in fungal biology · 2022Review
- Article
- Molecular Mechanisms Involved in the Multicellular Growth of Ustilaginomycetes.Microorganisms · 2020Review
- Comparative Genomic Data Provide New Insight on the Evolution of Pathogenicity inFrontiers in microbiology · 2020Article
- N-glycosylation of the protein disulfide isomerase Pdi1 ensures full Ustilago maydis virulence.PLoS pathogens · 2019Article
- Vps10-mediated targeting of Pep4 determines the activity of the vacuole in a substrate-dependent manner.Scientific reports · 2019Article
- Complementary Proteomics, Genomics approaches identifies potential pathogenicity/virulence factors in Tilletia indica induced under the influence of host factor.Scientific reports · 2019Article
- Heterologous expression and characterization of the aspartic endoprotease Pep4um from Ustilago maydis, a homolog of the human Chatepsin D, an important breast cancer therapeutic target.Molecular biology reports · 2018Article
- A Cinderella story: how the vacuolar proteases Pep4 and Prb1 do more than cleaning up the cell's mass degradation processes.Microbial cell (Graz, Austria) · 2018Review
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Vacuole proteases have important functions in different physiological processes in fungi. Taking this aspect into consideration, and as a continuation of our studies on the analysis of the proteolytic system of Ustilago maydis, a phytopathogenic member of the Basidiomycota, we have analysed the role of the pep4 gene encoding the vacuolar acid proteinase PrA in the pathogenesis and morphogenesis of the fungus. After confirmation of the location of the protease in the vacuole using fluorescent probes, we obtained deletion mutants of the gene in sexually compatible strains of U. maydis (FB1 and FB2), and analysed their phenotypes. It was observed that the yeast to mycelium dimorphic transition induced by a pH change in the medium, or the use of a fatty acid as sole carbon source, was severely reduced in Δpep4 mutants. In addition, the virulence of the mutants in maize seedlings was reduced, as revealed by the lower proportion of plants infected and the reduction in size of the tumours induced by the pathogen, when compared with wild-type strains. All of these phenotypic alterations were reversed by complementation of the mutant strains with the wild-type gene. These results provide evidence of the importance of the pep4 gene for the morphogenesis and virulence of U. maydis.
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