ArticleJournal of cellular physiology2015
Biphasic role of calcium in mouse sperm capacitation signaling pathways.
Article in Journal of cellular physiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 71 papers, 2 of them syntheses that pooled it.
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Who cites it
71 citing papers in PubMed, 2 syntheses or guidelines pooled it.
- The Catsper channel and its roles in male fertility: a systematic review.Reproductive biology and endocrinology : RB&E · 2017Pooled it
- Networks Models of Actin Dynamics during Spermatozoa Postejaculatory Life: A Comparison among Human-Made and Text Mining-Based Models.BioMed research international · 2016Pooled it
- The systems that transport ions and their impact on sperm function and male fertility.Function (Oxford, England) · 2026Review
- Effect of low-temperature stress on sperm DNA methylation and oxidative damage during cryopreservation of Tibetan pig semen.Veterinary world · 2026Article
- Incoherent feedforward loop dominates the robustness and tunability of necroptosis biphasic, emergent, and coexistent dynamics.Fundamental research · 2026Article
- CATSPERε extracellular domains are essential for sperm calcium channel assembly and activity modulation.Science advances · 2026Article
- Sperm capacitation in bulls, rams, bucks, and boars: molecular mechanisms and species-specific differences.Frontiers in veterinary science · 2026Review
- The impact of supplementation with calcium and naloxone to TRIS and Lepus extenders on the quality of refrigerated rabbit semen.Frontiers in veterinary science · 2026Article
- Beyond swimming: emerging parameters for predicting the fertility of mouse spermatozoa.Lab animal · 2025Review
- Coordinated and compartmentalized functions of CAII and CAIV establish a dual pH-regulatory mechanism essential for sperm maturation and capacitation.bioRxiv : the preprint server for biology · 2025Article
- Time and Media-Dependent Modulation of Sperm Capacitation in Red-Rumped Agouti (Dasyprocta leporina Linnaeus, 1758).Molecular reproduction and development · 2025Article
- Identification of sperm parameters linked to fertility and development of fertility prediction models for zebu (Bos indicus) and crossbred (Bos taurus X Bos indicus) cattle bulls.Tropical animal health and production · 2025Article
- Bovine serum albumin-induced calcium influx triggers soluble adenylyl cyclase activation and cyclic AMP signalling pathways in mouse sperm capacitation.The Journal of physiology · 2025Article
- Calcium Channels and Modulators as Potential Therapeutic Targets for Contraceptives and Male Fertility: A Scoping Review.Open access journal of contraception · 2025Review
- Increased reproductive outcomes after optimized sperm preparation.Frontiers in cell and developmental biology · 2025Article
- Advances in Molecular Biology and Immunology of Spermatozoa and Fertilization in Domestic Animals: Implications for Infertility and Assisted Reproduction.Current molecular medicine · 2025Review
- CATSPER2 and SPEF2 are potential molecular markers for boar sperm quality: a population association study.Journal of assisted reproduction and genetics · 2024Article
- Dual role of valosin-containing protein (VCP/p97) in mouse sperm during capacitation.Reproduction (Cambridge, England) · 2024Article
- CaAnimal cells and systems · 2024Review
- Identification of IQCH as a calmodulin-associated protein required for sperm motility in humans.iScience · 2023Article
11 more citing papers are in PubMed but not listed here.
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9 authors.
Funding
Abstract
Mammalian sperm acquire fertilizing ability in the female tract in a process known as capacitation. At the molecular level, capacitation is associated with up-regulation of a cAMP-dependent pathway, changes in intracellular pH, intracellular Ca(2+), and an increase in tyrosine phosphorylation. How these signaling systems interact during capacitation is not well understood. Results presented in this study indicate that Ca(2+) ions have a biphasic role in the regulation of cAMP-dependent signaling. Media without added Ca(2+) salts (nominal zero Ca(2+)) still contain micromolar concentrations of this ion. Sperm incubated in this medium did not undergo PKA activation or the increase in tyrosine phosphorylation suggesting that these phosphorylation pathways require Ca(2+). However, chelation of the extracellular Ca(2+) traces by EGTA induced both cAMP-dependent phosphorylation and the increase in tyrosine phosphorylation. The EGTA effect in nominal zero Ca(2+) media was mimicked by two calmodulin antagonists, W7 and calmidazolium, and by the calcineurin inhibitor cyclosporine A. These results suggest that Ca(2+) ions regulate sperm cAMP and tyrosine phosphorylation pathways in a biphasic manner and that some of its effects are mediated by calmodulin. Interestingly, contrary to wild-type mouse sperm, sperm from CatSper1 KO mice underwent PKA activation and an increase in tyrosine phosphorylation upon incubation in nominal zero Ca(2+) media. Therefore, sperm lacking Catsper Ca(2+) channels behave as wild-type sperm incubated in the presence of EGTA. This latter result suggests that Catsper transports the Ca(2+) involved in the regulation of cAMP-dependent and tyrosine phosphorylation pathways required for sperm capacitation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.