ArticleMetabolic engineering2015
Metabolic transistor strategy for controlling electron transfer chain activity in Escherichia coli.
Article in Metabolic engineering, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
8 citing papers in PubMed, 23 citations in OpenAlex.
- Reprogramming the metabolism ofSynthetic and systems biotechnology · 2021Article
- Dynamic control of the distribution of carbon flux between cell growth and butyrate biosynthesis in Escherichia coli.Applied microbiology and biotechnology · 2021Article
- Improved production of 2,3-butanediol and isobutanol by engineering electron transport chain in Escherichia coli.Microbial biotechnology · 2021Article
- Oxidative Stress Induction Is a Rational Strategy to Enhance the Productivity ofJournal of agricultural and food chemistry · 2020Article
- Metabolic Engineering of Bacterial Respiration: High vs. Low P/O and the Case ofFrontiers in bioengineering and biotechnology · 2019Review
- Strategies for manipulation of oxygen utilization by the electron transfer chain in microbes for metabolic engineering purposes.Journal of industrial microbiology & biotechnology · 2017Review
- Systematic manipulation of glutathione metabolism in Escherichia coli for improved glutathione production.Microbial cell factories · 2016Article
- ATP regulation in bioproduction.Microbial cell factories · 2015Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
Abstract
A novel strategy to finely control a large metabolic flux by using a "metabolic transistor" approach was established. In this approach a small change in the level or availability of an essential component for the process is controlled by adding a competitive reaction that affects a precursor or an intermediate in its biosynthetic pathway. The change of the basal level of the essential component, considered as a base current in a transistor, has a large effect on the flux through the major pathway. In this way, the fine-tuning of a large flux can be accomplished. The "metabolic transistor" strategy was applied to control electron transfer chain function by manipulation of the quinone synthesis pathway in Escherichia coli. The achievement of a theoretical yield of lactate production under aerobic conditions via this strategy upon manipulation of the biosynthetic pathway of the key participant, ubiquinone-8 (Q8), in an E. coli strain provides an in vivo, genetically tunable means to control the activity of the electron transfer chain and manipulate the production of reduced products while limiting consumption of oxygen to a defined amount.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.