ArticleBiology open2015
Highly efficient gene knockout by injection of TALEN mRNAs into oocytes and host transfer in Xenopus laevis.
Article in Biology open, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 23 citations in OpenAlex.
- How mRNA therapeutics are entering the monoclonal antibody field.Journal of translational medicine · 2019Review
- Oocyte Host-Transfer and Maternal mRNA Depletion Experiments inCold Spring Harbor protocols · 2018Article
- High-efficiency non-mosaic CRISPR-mediated knock-in and indel mutation in F0Development (Cambridge, England) · 2017Article
- Generation of a Xenopus laevis F1 albino J strain by genome editing and oocyte host-transfer.Developmental biology · 2017Article
- Expanding the genetic toolkit in Xenopus: Approaches and opportunities for human disease modeling.Developmental biology · 2017Review
- An optimized method for cryogenic storage of Xenopus sperm to maximise the effectiveness of research using genetically altered frogs.Theriogenology · 2017Article
- Oocyte shuttle, a recombinant protein transporting donor DNA into theBiology open · 2017Article
- Xenopus laevis as a model system to study cytoskeletal dynamics during axon pathfinding.Genesis (New York, N.Y. : 2000) · 2017Review
- A novel technique based on in vitro oocyte injection to improve CRISPR/Cas9 gene editing in zebrafish.Scientific reports · 2016Article
- Leapfrogging: primordial germ cell transplantation permits recovery of CRISPR/Cas9-induced mutations in essential genes.Development (Cambridge, England) · 2016Article
- Making muscle: Morphogenetic movements and molecular mechanisms of myogenesis in Xenopus laevis.Seminars in cell & developmental biology · 2016Review
- Using Xenopus laevis retinal and spinal neurons to study mechanisms of axon guidance in vivo and in vitro.Seminars in cell & developmental biology · 2016Review
- The Expression of TALEN before Fertilization Provides a Rapid Knock-Out Phenotype in Xenopus laevis Founder Embryos.PloS one · 2015Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Zinc-finger nucleases, transcription activator-like effector nucleases (TALENs) and the CRISPR/Cas (clustered regularly interspaced short palindromic repeats/CRISPR-associated proteins) system are potentially powerful tools for producing tailor-made knockout animals. However, their mutagenic activity is not high enough to induce mutations at all loci of a target gene throughout an entire tadpole. In this study, we present a highly efficient method for introducing gene modifications at almost all target sequences in randomly selected embryos. The gene modification activity of TALEN is enhanced by adopting the host-transfer technique. In our method, the efficiency is further improved by injecting TALEN mRNAs fused to the 3'UTR of the Xenopus DEADSouth gene into oocytes, which are then transferred into a host female frog, where they are ovulated and fertilized. The addition of the 3'UTR of the DEADSouth gene promotes mRNA translation in the oocytes and increases the expression of TALEN proteins to near-maximal levels three hours post fertilization (hpf). In contrast, TALEN mRNAs without this 3'UTR are translated infrequently in oocytes. Our data suggest that genomic DNA is more sensitive to TALEN proteins from fertilization to the midblastula (MBT) stage. Our method works by increasing the levels of TALEN proteins during the pre-MBT stages.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.