ArticleBiology open2015
A proteomic study of mitotic phase-specific interactors of EB1 reveals a role for SXIP-mediated protein interactions in anaphase onset.
Article in Biology open, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 35 citations in OpenAlex.
- Dysregulation of the Tau-Microtubule-End-Binding Protein Axis in Alzheimer's Disease and Related Tauopathies.International journal of molecular sciences · 2026Review
- SKAP binding to microtubules reduces friction at the kinetochore-microtubule interface and increases attachment stability under force.Current biology : CB · 2025Article
- SKAP binding to microtubules reduces friction at the kinetochore-microtubule interface and increases attachment stability under force.bioRxiv : the preprint server for biology · 2024Article
- CKAP5 stabilizes CENP-E at kinetochores by regulating microtubule-chromosome attachments.EMBO reports · 2024Article
- Article
- Deep learning techniques and mathematical modeling allow 3D analysis of mitotic spindle dynamics.The Journal of cell biology · 2023Article
- The Astrin-SKAP complex reduces friction at the kinetochore-microtubule interface.Current biology : CB · 2022Article
- Counteraction between Astrin-PP1 and Cyclin-B-CDK1 pathways protects chromosome-microtubule attachments independent of biorientation.Nature communications · 2021Article
- Article
- MARK2/Par1b kinase present at centrosomes and retraction fibres corrects spindle off-centring induced by actin disassembly.Open biology · 2019Article
- Mapping the kinetochore MAP functions required for stabilizing microtubule attachments to chromosomes during metaphase.Cytoskeleton (Hoboken, N.J.) · 2019Review
- Quantitative analysis of sensitivity to a Wnt3a gradient in determination of the pole-to-pole axis of mitotic cells by using a microfluidic device.FEBS open bio · 2018Article
- Spindle rotation in human cells is reliant on a MARK2-mediated equatorial spindle-centering mechanism.The Journal of cell biology · 2018Article
- Two populations of cytoplasmic dynein contribute to spindle positioning inThe Journal of cell biology · 2017Article
- Astrin-SKAP complex reconstitution reveals its kinetochore interaction with microtubule-bound Ndc80.eLife · 2017Article
- Aurora-B kinase pathway controls the lateral to end-on conversion of kinetochore-microtubule attachments in human cells.Nature communications · 2017Article
- Regulation of end-binding protein EB1 in the control of microtubule dynamics.Cellular and molecular life sciences : CMLS · 2017Review
- Phosphorylation of SKAP by GSK3β ensures chromosome segregation by a temporal inhibition of Kif2b activity.Scientific reports · 2016Article
- An unconventional interaction between Dis1/TOG and Mal3/EB1 in fission yeast promotes the fidelity of chromosome segregation.Journal of cell science · 2016Article
- A mitotic SKAP isoform regulates spindle positioning at astral microtubule plus ends.The Journal of cell biology · 2016Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Microtubules execute diverse mitotic events that are spatially and temporally separated; the underlying regulation is poorly understood. By combining drug treatments, large-scale immunoprecipitation and mass spectrometry, we report the first comprehensive map of mitotic phase-specific protein interactions of the microtubule-end binding protein, EB1. EB1 interacts with some, but not all, of its partners throughout mitosis. We show that the interaction of EB1 with Astrin-SKAP complex, a key regulator of chromosome segregation, is enhanced during prometaphase, compared to anaphase. We find that EB1 and EB3, another EB family member, can interact directly with SKAP, in an SXIP-motif dependent manner. Using an SXIP defective mutant that cannot interact with EB, we uncover two distinct pools of SKAP at spindle microtubules and kinetochores. We demonstrate the importance of SKAP's SXIP-motif in controlling microtubule growth rates and anaphase onset, without grossly disrupting spindle function. Thus, we provide the first comprehensive map of temporal changes in EB1 interactors during mitosis and highlight the importance of EB protein interactions in ensuring normal mitosis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.