ArticleApplied and environmental microbiology2015
Bacteriophage PBC1 and its endolysin as an antimicrobial agent against Bacillus cereus.
Article in Applied and environmental microbiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers, 1 of them a synthesis that pooled it.
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Who cites it
43 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Pooled it
- Antimicrobial Resistance Profiles, Resistance Genes, and Their Association with Bacteriophage Susceptibility AmongMicroorganisms · 2026Article
- Bacteriophage BCP01 and Its Endolysin as Tools for Biocontrol and Rapid Detection ofJournal of microbiology and biotechnology · 2026Article
- Characterization of Endolysin LysG77YL fromJournal of microbiology and biotechnology · 2026Article
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- Characterisation of a Novel Jumbo Lytic Aeromonas dhakensis Bacteriophage P19 and Its Endolysin.Microbial biotechnology · 2026Article
- Phage Therapy forAntibiotics (Basel, Switzerland) · 2025Review
- Genomic and Biological Insights of Bacteriophage ΦBc24 TargetingCurrent issues in molecular biology · 2025Article
- Expression and application of a novel Holin Hol6A3 in phage PD6A3 of Acinetobacter baumannii.BMC microbiology · 2025Article
- Phage Endolysins as an Alternative Biocontrol Strategy for Pathogenic and Spoilage Microorganisms in the Food Industry.Viruses · 2025Review
- Isolation and Characterization of aJournal of microbiology and biotechnology · 2025Article
- Characterization ofAntibiotics (Basel, Switzerland) · 2025Article
- Limitation of the Lytic Effect of Bacteriophages onInternational journal of microbiology · 2025Review
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- Characterization of a Putative Lysin from Enterococcus faecalis Phage IME-EFm1 and Determination of its Protective Efficacy in Mice.Current microbiology · 2024Article
- Characterization of the novel bequatrovirus vB-BcgM and its antibacterial effects in a food matrix.Archives of virology · 2024Article
- Agents Targeting the Bacterial Cell Wall as Tools to Combat Gram-Positive Pathogens.Molecules (Basel, Switzerland) · 2024Review
- Article
- Complete Genome Sequence of the Bacillus cereus Temperate Bacteriophage BSG01.Microbiology resource announcements · 2023Article
- Phage Adsorption to Gram-Positive Bacteria.Viruses · 2023Review
Corrections and comments
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Bacillus cereus is an opportunistic human pathogen responsible for food poisoning and other, nongastrointestinal infections. Due to the emergence of multidrug-resistant B. cereus strains, the demand for alternative therapeutic options is increasing. To address these problems, we isolated and characterized a Siphoviridae virulent phage, PBC1, and its lytic enzymes. PBC1 showed a very narrow host range, infecting only 1 of 22 B. cereus strains. Phylogenetic analysis based on the major capsid protein revealed that PBC1 is more closely related to the Bacillus clarkii phage BCJA1c and phages of lactic acid bacteria than to the phages infecting B. cereus. Whole-genome comparison showed that the late-gene region, including the terminase gene, structural genes, and holin gene of PBC1, is similar to that from B. cereus temperate phage 250, whereas their endolysins are different. Compared to the extreme host specificity of PBC1, its endolysin, LysPBC1, showed a much broader lytic spectrum, albeit limited to the genus Bacillus. The catalytic domain of LysPBC1 when expressed alone also showed Bacillus-specific lytic activity, which was lower against the B. cereus group but higher against the Bacillus subtilis group than the full-length protein. Taken together, these results suggest that the virulent phage PBC1 is a useful component of a phage cocktail to control B. cereus, even with its exceptionally narrow host range, as it can kill a strain of B. cereus that is not killed by other phages, and that LysPBC1 is an alternative biocontrol agent against B. cereus.
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