ArticleApplied and environmental microbiology2015
Metabolic engineering of Corynebacterium glutamicum for methanol metabolism.
Article in Applied and environmental microbiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed, 107 citations in OpenAlex.
- Repair of DNA and protein damages caused by formaldehyde improves methanol assimilation.Fundamental research · 2026Article
- Tailoring Corynebacterium glutamicum for Sustainable Biomanufacturing: From Traditional to Cutting-Edge Technologies.Molecular biotechnology · 2026Review
- Industrial applicability of enzymatic and whole-cell processes for the utilization of C1 building blocks.Nature communications · 2025Review
- EngineeringBiodesign research · 2025Review
- A synthetic methylotroph achieves accelerated cell growth by alleviating transcription-replication conflicts.Nature communications · 2025Article
- Single carbon metabolism - A new paradigm for microbial bioprocesses?Synthetic and systems biotechnology · 2024Review
- A synthetic methylotrophicNature catalysis · 2024Article
- Metabolic engineering strategies for microbial utilization of methanol.Engineering microbiology · 2023Review
- Methanol-based biomanufacturing of fuels and chemicals using native and synthetic methylotrophs.Synthetic and systems biotechnology · 2023Article
- Polymers without Petrochemicals: Sustainable Routes to Conventional Monomers.Chemical reviews · 2023Review
- Design and Construction of Artificial Biological Systems for One-Carbon Utilization.Biodesign research · 2023Review
- Synthetic methylotrophic yeasts for the sustainable fuel and chemical production.Biotechnology for biofuels and bioproducts · 2022Review
- Yeast transcriptional device libraries enable precise synthesis of value-added chemicals from methanol.Nucleic acids research · 2022Article
- Toward Methanol-Based Biomanufacturing: Emerging Strategies for Engineering Synthetic Methylotrophy inACS synthetic biology · 2022Review
- Unravelling Formaldehyde Metabolism in Bacteria: Road towards Synthetic Methylotrophy.Microorganisms · 2022Review
- Developing methylotrophic microbial platforms for a methanol-based bioindustry.Frontiers in bioengineering and biotechnology · 2022Review
- Bioconversion of Methanol by Synthetic Methylotrophy.Advances in biochemical engineering/biotechnology · 2022Article
- Developing Synthetic Methylotrophs by Metabolic Engineering-Guided Adaptive Laboratory Evolution.Advances in biochemical engineering/biotechnology · 2022Article
- From a Hetero- to a Methylotrophic Lifestyle: Flash Back on the Engineering Strategies to Create Synthetic Methanol-User Strains.Frontiers in bioengineering and biotechnology · 2022Review
- Rewiring the microbial metabolic network for efficient utilization of mixed carbon sources.Journal of industrial microbiology & biotechnology · 2021Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Methanol is already an important carbon feedstock in the chemical industry, but it has found only limited application in biotechnological production processes. This can be mostly attributed to the inability of most microbial platform organisms to utilize methanol as a carbon and energy source. With the aim to turn methanol into a suitable feedstock for microbial production processes, we engineered the industrially important but nonmethylotrophic bacterium Corynebacterium glutamicum toward the utilization of methanol as an auxiliary carbon source in a sugar-based medium. Initial oxidation of methanol to formaldehyde was achieved by heterologous expression of a methanol dehydrogenase from Bacillus methanolicus, whereas assimilation of formaldehyde was realized by implementing the two key enzymes of the ribulose monophosphate pathway of Bacillus subtilis: 3-hexulose-6-phosphate synthase and 6-phospho-3-hexuloisomerase. The recombinant C. glutamicum strain showed an average methanol consumption rate of 1.7 ± 0.3 mM/h (mean ± standard deviation) in a glucose-methanol medium, and the culture grew to a higher cell density than in medium without methanol. In addition, [(13)C]methanol-labeling experiments revealed labeling fractions of 3 to 10% in the m + 1 mass isotopomers of various intracellular metabolites. In the background of a C. glutamicum Δald ΔadhE mutant being strongly impaired in its ability to oxidize formaldehyde to CO2, the m + 1 labeling of these intermediates was increased (8 to 25%), pointing toward higher formaldehyde assimilation capabilities of this strain. The engineered C. glutamicum strains represent a promising starting point for the development of sugar-based biotechnological production processes using methanol as an auxiliary substrate.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.