ArticleApplied and environmental microbiology2015
Counting viruses and bacteria in photosynthetic microbial mats.
Article in Applied and environmental microbiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 34 citations in OpenAlex.
- High-throughput viral enumeration of aquatic ecosystems via flow cytometry.Applied and environmental microbiology · 2026Article
- Exploring the prokaryote-eukaryote interplay in microbial mats from an Andean athalassohaline wetland.Microbiology spectrum · 2024Article
- Viral enumeration using cost-effective wet-mount epifluorescence microscopy for aquatic ecosystems and modern microbialites.Applied and environmental microbiology · 2023Article
- Unique Geothermal Chemistry Shapes Microbial Communities on Mt. Erebus, Antarctica.Frontiers in microbiology · 2022Article
- An Advanced Protocol for the Quantification of Marine Sediment Viruses via Flow Cytometry.Viruses · 2021Article
- Widespread cryptic viral infections in lotic biofilms.Biofilm · 2020Article
- Epigenetic biosensors for bacteriophage detection and phage receptor discrimination.Environmental microbiology · 2020Article
- Mesophilic microorganisms build terrestrial mats analogous to Precambrian microbial jungles.Nature communications · 2019Article
- Active microbiome structure and its association with environmental factors and viruses at different aquatic sites of a high-altitude wetland.MicrobiologyOpen · 2019Article
- Abundance and Distribution of Microbial Cells and Viruses in an Alluvial Aquifer.Frontiers in microbiology · 2017Article
- Seasonal Dynamics and Metagenomic Characterization of Marine Viruses in Goseong Bay, Korea.PloS one · 2017Article
- Abundance and Distribution of Enteric Bacteria and Viruses in Coastal and Estuarine Sediments-a Review.Frontiers in microbiology · 2016Review
- Enumerating Virus-Like Particles and Bacterial Populations in the Sinuses of Chronic Rhinosinusitis Patients Using Flow Cytometry.PloS one · 2016Article
- Microscale spatial distributions of microbes and viruses in intertidal photosynthetic microbial mats.SpringerPlus · 2015Article
- Viral Impact on Prokaryotic and Microalgal Activities in the Microphytobenthic Biofilm of an Intertidal Mudflat (French Atlantic Coast).Frontiers in microbiology · 2015Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Viral abundances in benthic environments are the highest found in aquatic systems. Photosynthetic microbial mats represent benthic environments with high microbial activity and possibly high viral densities, yet viral abundances have not been examined in such systems. Existing extraction procedures typically used in benthic viral ecology were applied to the complex matrix of microbial mats but were found to inefficiently extract viruses. Here, we present a method for extraction and quantification of viruses from photosynthetic microbial mats using epifluorescence microscopy (EFM) and flow cytometry (FCM). A combination of EDTA addition, probe sonication, and enzyme treatment applied to a glutaraldehyde-fixed sample resulted in a substantially higher viral (5- to 33-fold) extraction efficiency and reduced background noise compared to previously published methods. Using this method, it was found that in general, intertidal photosynthetic microbial mats harbor very high viral abundances (2.8 × 10(10) ± 0.3 × 10(10) g(-1)) compared with benthic habitats (10(7) to 10(9) g(-1)). This procedure also showed 4.5- and 4-fold-increased efficacies of extraction of viruses and bacteria, respectively, from intertidal sediments, allowing a single method to be used for the microbial mat and underlying sediment.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.