ArticleApplied and environmental microbiology2015
Improved production of propionic acid in Propionibacterium jensenii via combinational overexpression of glycerol dehydrogenase and malate dehydrogenase from Klebsiella pneumoniae.
Article in Applied and environmental microbiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 52 citations in OpenAlex.
- Metabolic marker-assisted genomic prediction improves hybrid breeding.Plant communications · 2025Article
- Bacteria and bacteriophage consortia are associated with protective intestinal metabolites in patients receiving stem cell transplantation.Nature cancer · 2024Article
- Fermentative production of propionic acid: prospects and limitations of microorganisms and substrates.Applied microbiology and biotechnology · 2021Review
- Oxidation of 1-propanol to propionic acid with hydrogen peroxide catalysed by heteropolyoxometalates.BMC chemistry · 2021Article
- Article
- Microbial response to acid stress: mechanisms and applications.Applied microbiology and biotechnology · 2020Review
- Genome-scale model guided design ofMetabolic engineering communications · 2018Article
- Propionibacterium spp.-source of propionic acid, vitamin B12, and other metabolites important for the industry.Applied microbiology and biotechnology · 2018Review
- Cofactor recycling for co-production of 1,3-propanediol and glutamate by metabolically engineered Corynebacterium glutamicum.Scientific reports · 2017Article
- Production of acrylic acid and propionic acid by constructing a portion of the 3-hydroxypropionate/4-hydroxybutyrate cycle from Metallosphaera sedula in Escherichia coli.Journal of industrial microbiology & biotechnology · 2016Article
- Pathway engineering of Propionibacterium jensenii for improved production of propionic acid.Scientific reports · 2016Article
- Microbial Production of Short Chain Fatty Acids from Lignocellulosic Biomass: Current Processes and Market.BioMed research international · 2016Review
- Key enzymes catalyzing glycerol to 1,3-propanediol.Biotechnology for biofuels · 2016Review
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Microbial production of propionic acid (PA), an important chemical building block used as a preservative and chemical intermediate, has gained increasing attention for its environmental friendliness over traditional petrochemical processes. In previous studies, we constructed a shuttle vector as a useful tool for engineering Propionibacterium jensenii, a potential candidate for efficient PA synthesis. In this study, we identified the key metabolites for PA synthesis in P. jensenii by examining the influence of metabolic intermediate addition on PA synthesis with glycerol as a carbon source under anaerobic conditions. We also further improved PA production via the overexpression of the identified corresponding enzymes, namely, glycerol dehydrogenase (GDH), malate dehydrogenase (MDH), and fumarate hydratase (FUM). Compared to those in wild-type P. jensenii, the activities of these enzymes in the engineered strains were 2.91- ± 0.17- to 8.12- ± 0.37-fold higher. The transcription levels of the corresponding enzymes in the engineered strains were 2.85- ± 0.19- to 8.07- ± 0.63-fold higher than those in the wild type. The coexpression of GDH and MDH increased the PA titer from 26.95 ± 1.21 g/liter in wild-type P. jensenii to 39.43 ± 1.90 g/liter in the engineered strains. This study identified the key metabolic nodes limiting PA overproduction in P. jensenii and further improved PA titers via the coexpression of GDH and MDH, making the engineered P. jensenii strain a potential industrial producer of PA.
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