ArticleParasites & vectors2015
Leishmania enriettii: biochemical characterisation of lipophosphoglycans (LPGs) and glycoinositolphospholipids (GIPLs) and infectivity to Cavia porcellus.
Article in Parasites & vectors, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 31 citations in OpenAlex.
- Phenotypical Differences betweenPathogens (Basel, Switzerland) · 2023Article
- C-reactive protein binds to short phosphoglycan repeats ofFrontiers in immunology · 2023Article
- Leishmania amazonensis from distinct clinical forms/hosts has polymorphisms in Lipophosphoglycans, displays variations in immunomodulatory properties and, susceptibility to antileishmanial drugs.Cell biology international · 2022Article
- Leishmania enriettii visceralises in the trachea, lungs, and spleen of Cavia porcellus.Memorias do Instituto Oswaldo Cruz · 2022Article
- Lipophosphoglycan From Dermotropic New WorldFrontiers in cellular and infection microbiology · 2022Article
- Genomics of Trypanosomatidae: Where We Stand and What Needs to Be Done?Pathogens (Basel, Switzerland) · 2021Review
- Insights intoVeterinary sciences · 2021Review
- Article
- Lipophosphoglycans from dermotropic Leishmania infantum are more pro-inflammatory than those from viscerotropic strains.Memorias do Instituto Oswaldo Cruz · 2020Article
- Intraspecies Polymorphisms in the Lipophosphoglycan ofFrontiers in cellular and infection microbiology · 2019Article
- Glycoconjugates of Gram-negative bacteria and parasitic protozoa - are they similar in orchestrating the innate immune response?Innate immunity · 2019Review
- The LPG1x family from Leishmania major is constituted of rare eukaryotic galactofuranosyltransferases with unprecedented catalytic properties.Scientific reports · 2018Article
- Salivary Gland Extract Modulates the Infection of TwoFrontiers in microbiology · 2018Article
- Leishmania infantum lipophosphoglycan induced-Prostaglandin EScientific reports · 2017Article
- Leishmania enriettii (Muniz & Medina, 1948): A highly diverse parasite is here to stay.PLoS pathogens · 2017Review
- Lipophosphoglycans from Leishmania amazonensis Strains Display Immunomodulatory Properties via TLR4 and Do Not Affect Sand Fly Infection.PLoS neglected tropical diseases · 2016Article
- The Biting Midge Culicoides sonorensis (Diptera: Ceratopogonidae) Is Capable of Developing Late Stage Infections of Leishmania enriettii.PLoS neglected tropical diseases · 2015Article
- Vesicles from different Trypanosoma cruzi strains trigger differential innate and chronic immune responses.Journal of extracellular vesicles · 2015Article
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Authors and funding
13 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundLeishmania enriettii is a species non-infectious to man, whose reservoir is the guinea pig Cavia porcellus. Many aspects of the parasite-host interaction in this model are unknown, especially those involving parasite surface molecules. While lipophosphoglycans (LPGs) and glycoinositolphospholipids (GIPLs) of Leishmania species from the Old and New World have already been described, glycoconjugates of L. enriettii and their importance are still unknown.
methodsMice peritoneal macrophages from C57BL/6 and knock-out (TLR2 -/-, TLR4 -/-) were primed with IFN-γ and stimulated with purified LPG and GIPLs from both species. Nitric oxide and cytokine production were performed. MAPKs (p38 and JNK) and NF-kB activation were evaluated in J774.1 macrophages and CHO cells, respectively.
resultsLPGs were extracted, purified and analysed by western-blot, showing that LPG from L88 strain was longer than that of Cobaia strain. LPGs and GIPLs were depolymerised and their sugar content was determined. LPGs from both strains did not present side chains, having the common disaccharide Gal(β1,4)Man(α1)-PO4. The GIPL from L88 strain presented galactose in its structure, suggestive of type II GIPL. On the other hand, the GIPL of Cobaia strain presented an abundance of glucose, a characteristic not previously observed. Mice peritoneal macrophages from C57BL/6 and knock-outs (TLR2 -/- and TLR4 -/-) were primed with IFN-γ and stimulated with glycoconjugates and live parasites. No activation of NO or cytokines was observed with live parasites. On the other hand, LPGs and GIPLs were able to activate the production of NO, IL-6, IL-12 and TNF-α preferably via TRL2. However, in CHO cells, only GIPLs were able to activate TRL2 and TRL4. In vivo studies using male guinea pigs (Cavia porcellus) showed that only strain L88 was able to develop more severe ulcerated lesions especially in the presence of salivary gland extract (SGE).
conclusionThe two L. enriettii strains exhibited polymorphisms in their LPGs and GIPLs and those features may be related to a more pro-inflammatory profile in the L88 strain.
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