Evidence map›Paper›PMID 25594326›Full record

ArticleJournal of neurosurgery2015

Effect of exosomes derived from multipluripotent mesenchymal stromal cells on functional recovery and neurovascular plasticity in rats after traumatic brain injury.

Yanlu Zhang, Michael Chopp, Yuling Meng, Mark Katakowski, Hongqi Xin, Asim Mahmood, Ye Xiong

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of neurosurgery, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 426 papers, 4 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
426citing papers in PubMed, 4 pooled it
25.2field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

426 citing papers in PubMed, 4 syntheses or guidelines pooled it, 736 citations in OpenAlex.

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366 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Yanlu ZhangDepartments of 1 Neurosurgery and.
Michael Chopp
Yuling Meng
Mark Katakowski
Hongqi Xin
Asim Mahmood
Ye Xiong
Henry Ford Hospital · US

Funding

Exosome transfer of miR-133b mediates MSC induced neurological recovery after strR01NS081189 · NINDS · HENRY FORD HEALTH SYSTEM · PI XIN, HONGQI · 2013 to 2016
$1.2M
NINDS NIH HHS R01 NS081189NINDS NIH HHS R01NS081189
6 · The paper itself

Abstract

objectTransplanted multipotent mesenchymal stromal cells (MSCs) improve functional recovery in rats after traumatic brain injury (TBI). In this study the authors tested a novel hypothesis that systemic administration of cell-free exosomes generated from MSCs promotes functional recovery and neurovascular remodeling in rats after TBI.

methodsTwo groups of 8 Wistar rats were subjected to TBI, followed 24 hours later by tail vein injection of 100 μg protein of exosomes derived from MSCs or an equal volume of vehicle (phosphate-buffered saline). A third group of 8 rats was used as sham-injured, sham-treated controls. To evaluate cognitive and sensorimotor functional recovery, the modified Morris water maze, modified Neurological Severity Score, and foot-fault tests were performed. Animals were killed at 35 days after TBI. Histopathological and immunohistochemical analyses were performed for measurements of lesion volume, neurovascular remodeling (angiogenesis and neurogenesis), and neuroinflammation.

resultsCompared with the saline-treated group, exosome-treated rats with TBI showed significant improvement in spatial learning at 34-35 days as measured by the modified Morris water maze test (p < 0.05), and sensorimotor functional recovery (i.e., reduced neurological deficits and foot-fault frequency) was observed at 14-35 days postinjury (p < 0.05). Exosome treatment significantly increased the number of newly generated endothelial cells in the lesion boundary zone and dentate gyrus and significantly increased the number of newly formed immature and mature neurons in the dentate gyrus as well as reducing neuroinflammation.

conclusionsThe authors demonstrate for the first time that MSC-generated exosomes effectively improve functional recovery, at least in part, by promoting endogenous angiogenesis and neurogenesis and by reducing inflammation in rats after TBI. Thus, MSC-generated exosomes may provide a novel cell-free therapy for TBI and possibly for other neurological diseases.

Indexed as

Mesenchymal Stem CellsNeuronal PlasticityAnimalsBlood VesselsBrain InjuriesCell CountCognitionDoublecortin ProteinExosomesInjections, IntravenousMaleMaze LearningMesenchymal Stem Cell TransplantationPluripotent Stem CellsRatsRats, WistarDoublecortin ProteinangiogenesisBrdU = 5-bromo-2′-deoxyuridineDCX = doublecortinDG = dentate gyrusEBA = endothelial barrier antigenexosomesFBS = fetal bovine serumfunctional recoveryGFAP = glial fibrillary acidic proteinGFP = green fluorescent proteinmesenchymal stromal cellmiRNA = microRNAmNSS = modified Neurological Severity ScoreMSC = mesenchymal stromal cellMWM = Morris Water MazeNeuN = neuron-specific nuclear proteinneurogenesisPBS = phosphate-buffered salineTBI = traumatic brain injurytraumatic brain injury

Identifiers

PMID25594326
PMCPMC4382456
OpenAlexW2168120562

What OpenQuestion holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.