Evidence map›Paper›PMID 25593511›Full record

ArticleMolecular vision2015

Transcriptional regulation of crystallin, redox, and apoptotic genes by C-Phycocyanin in the selenite-induced cataractogenic rat model.

Rasiah Pratheepa Kumari, Srinivasagan Ramkumar, Bency Thankappan, Kalimuthusamy Natarajaseenivasan, Sadhasivam Balaji, Kumarasamy Anbarasu

Abstract read
PubMed Full text at PMC
In one paragraph

Article in Molecular vision, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
1.7field-weighted citation impact, top 17% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed, 25 citations in OpenAlex.

  1. Review
  2. Article
  3. Article
  4. Polyphenol-enriched fraction ofMolecular vision · 2019
    Article
  5. Review
  6. The impact of miR-26b on retinal pigment epithelium cells in rhegmatogenous retinal detachment model.International journal of clinical and experimental pathology · 2017
    Article
  7. Molecular vision · 2017
    Article
  8. Medical Application of Spirulina platensis Derived C-Phycocyanin.Evidence-based complementary and alternative medicine : eCAM · 2016
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Rasiah Pratheepa KumariDepartment of Marine Biotechnology, Bharathidasan University, Tiruchirappalli, Tamil Nadu, India.
Srinivasagan RamkumarDepartment of Marine Biotechnology, Bharathidasan University, Tiruchirappalli, Tamil Nadu, India.
Bency ThankappanDepartment of Marine Biotechnology, Bharathidasan University, Tiruchirappalli, Tamil Nadu, India.
Kalimuthusamy NatarajaseenivasanDepartment of Microbiology, School of Life Sciences, Bharathidasan University, Tiruchirappalli, Tamil Nadu, India.
Sadhasivam BalajiDepartment of Endocrinology, Dr. A. L. M. PG IBMS, University of Madras, Chennai, Tamil Nadu, India.
Kumarasamy AnbarasuDepartment of Marine Biotechnology, Bharathidasan University, Tiruchirappalli, Tamil Nadu, India.
Bharathidasan University · IN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

purposeThis study was designed to examine the constrictive potential of C-Phycocyanin (C-PC) in regulating changes imposed on gene expression in the selenite-induced cataract model.

methodsWistar rat pups were divided into three groups of eight each. On P10, Group I received an intraperitoneal injection of normal saline. Groups II and III received a subcutaneous injection of sodium selenite (19 μmol/kg bodyweight); Group III also received an intraperitoneal injection of C-PC (200 mg/kg bodyweight) on P9-14. Total RNA was isolated on P16, and the relative abundance of mRNA of the crystallin structural genes, redox components, and apoptotic cascade were ascertained with real-time PCR with reference to the internal control β-actin.

resultsReal-time PCR analysis showed the crystallin genes (αA-, βB1-, γD-) and redox cycle components (Cat, SOD-1, Gpx) were downregulated, the apoptotic components were upregulated, and antiapoptotic Bcl-2 was downregulated in Group II. Treatment with 200 mg/kg bodyweight C-PC (Group III) transcriptionally regulated the instability of the expression of these genes, thus ensuring C-PC is a prospective anticataractogenic agent that probably delays the onset and progression of cataractogenesis induced by sodium selenite.

conclusionsC-PC treatment possibly prevented cataractogenesis triggered by sodium selenite, by regulating the lens crystallin, redox genes, and apoptotic cascade mRNA expression and thus maintains lens transparency. C-PC may be developed as a potential antioxidant compound applied in the future to prevent and treat age-related cataract.

Indexed as

AnimalsAnimals, NewbornCatalaseCataractCrystallinsDisease Models, AnimalGene Expression RegulationGlutathione PeroxidaseInjections, IntraperitonealLens, CrystallineMicrotubule-Associated ProteinsPhycocyaninProtective AgentsProtein IsoformsProto-Oncogene Proteins c-bcl-2RatsCatalasecryaB protein, ratCrystallinsGlutathione PeroxidaseMicrotubule-Associated ProteinsPhycocyaninProtective AgentsProtein IsoformsProto-Oncogene Proteins c-bcl-2RNA, MessengerSelenious AcidSuperoxide Dismutase

Identifiers

PMID25593511
PMCPMC4301595
OpenAlexW1516279818

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.