ArticleThe Journal of biological chemistry2015
Na+ transport by the A1AO-ATP synthase purified from Thermococcus onnurineus and reconstituted into liposomes.
Article in The Journal of biological chemistry, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 21 citations in OpenAlex.
- Diversity of Archaea.Progress in molecular and subcellular biology · 2026Review
- Formate-driven HBiotechnology for biofuels and bioproducts · 2022Article
- ATP synthesis in an ancient ATP synthase at low driving forces.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- HThe ISME journal · 2021Article
- The Rnf complex is a NaCommunications biology · 2020Article
- Thermococcus indicus sp. nov., a Fe(III)-reducing hyperthermophilic archaeon isolated from the Onnuri Vent Field of the Central Indian Ocean ridge.Journal of microbiology (Seoul, Korea) · 2020Article
- Energy-converting hydrogenases: the link between HCellular and molecular life sciences : CMLS · 2020Review
- Overcoming Energetic Barriers in Acetogenic C1 Conversion.Frontiers in bioengineering and biotechnology · 2020Review
- Energy conservation by a hydrogenase-dependent chemiosmotic mechanism in an ancient metabolic pathway.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The ATP synthase of many archaea has the conserved sodium ion binding motif in its rotor subunit, implying that these A1AO-ATP synthases use Na(+) as coupling ion. However, this has never been experimentally verified with a purified system. To experimentally address the nature of the coupling ion, we have purified the A1AO-ATP synthase from T. onnurineus. It contains nine subunits that are functionally coupled. The enzyme hydrolyzed ATP, CTP, GTP, UTP, and ITP with nearly identical activities of around 40 units/mg of protein and was active over a wide pH range with maximal activity at pH 7. Noteworthy was the temperature profile. ATP hydrolysis was maximal at 80 °C and still retained an activity of 2.5 units/mg of protein at 45 °C. The high activity of the enzyme at 45 °C opened, for the first time, a way to directly measure ion transport in an A1AO-ATP synthase. Therefore, the enzyme was reconstituted into liposomes generated from Escherichia coli lipids. These proteoliposomes were still active at 45 °C and coupled ATP hydrolysis to primary and electrogenic Na(+) transport. This is the first proof of Na(+) transport by an A1AO-ATP synthase and these findings are discussed in light of the distribution of the sodium ion binding motif in archaea and the role of Na(+) in the bioenergetics of archaea.
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