ArticleMicrobial cell factories2015
High crude violacein production from glucose by Escherichia coli engineered with interactive control of tryptophan pathway and violacein biosynthetic pathway.
Article in Microbial cell factories, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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41 citing papers in PubMed, 97 citations in OpenAlex.
- Hybrid metabolic engineering enables xylose-driven co-production of polyhydroxybutyrate and violacein in Escherichia coli.Journal of biological engineering · 2026Article
- Bioconversion of a Dairy By-Product (Microorganisms · 2025Article
- A comprehensive review on violacein production by microbial fermentation.Biodesign research · 2025Review
- Investigating the Potential of Division of Labor in Synthetic Bacterial Communities for the Production of Violacein.ACS synthetic biology · 2025Article
- Production, downstream, stability, bioactivities, and applications studies of violacein and related compounds.World journal of microbiology & biotechnology · 2025Review
- Engineering Escherichia coli for Efficient Production of L-Tryptophan.Applied biochemistry and biotechnology · 2025Article
- Design principles for engineering bacteria to maximise chemical production from batch cultures.Nature communications · 2025Article
- Stability of a MutualisticEngineering in life sciences · 2024Article
- Pigments from pathogenic bacteria: a comprehensive update on recent advances.World journal of microbiology & biotechnology · 2024Review
- Synthetic co-culture in an interconnected two-compartment bioreactor system: violacein production with recombinant E. coli strains.Bioprocess and biosystems engineering · 2024Article
- Article
- Evaluation and comparison of colorimetric outputs for yeast-based biosensors in laboratory and point-of-use settings.FEMS microbiology letters · 2024Article
- Purification of Natural Pigments Violacein and Deoxyviolacein Produced by Fermentation UsingMolecules (Basel, Switzerland) · 2023Article
- New Target Gene Screening Using Shortened and Random sgRNA Libraries in Microbial CRISPR Interference.ACS synthetic biology · 2023Article
- Expanding the application of tryptophan: Industrial biomanufacturing of tryptophan derivatives.Frontiers in microbiology · 2023Review
- The modular pYT vector series employed for chromosomal gene integration and expression to produce carbazoles and glycolipids inFEMS microbes · 2023Article
- Microbial pigments: Learning from Himalayan perspective to industrial applications.Journal of industrial microbiology & biotechnology · 2022Article
- Improvement in Violacein Production by Utilizing Formic Acid to Induce Quorum Sensing inAntioxidants (Basel, Switzerland) · 2022Article
- Developing methylotrophic microbial platforms for a methanol-based bioindustry.Frontiers in bioengineering and biotechnology · 2022Review
- Recent Advances in Synthetic, Industrial and Biological Applications of Violacein and Its Heterologous Production.Journal of microbiology and biotechnology · 2021Review
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Authors and funding
8 authors at 5 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundAs bacteria-originated crude violacein, a natural indolocarbazole product, consists of violacein and deoxyviolacein, and can potentially be a new type of natural antibiotics, the reconstruction of an effective metabolic pathway for crude violacein (violacein and deoxyviolacein mixture) synthesis directly from glucose in Escherichia coli was of importance for developing industrial production process.
resultsStrains with a multivariate module for varied tryptophan productivities were firstly generated by combinatorial knockout of trpR/tnaA/pheA genes and overexpression of two key genes trpEfbr /trpD from the upstream tryptophan metabolic pathway. Then, the gene cluster of violacein biosynthetic pathway was introduced downstream of the generated tryptophan pathway. After combination of these two pathways, maximum crude violacein production directly from glucose by E. coli B2/pED+pVio was realized with a titer of 0.6±0.01 g L(-1) in flask culture, which was four fold higher than that of the control without the tryptophan pathway up-regulation. In a 5-L bioreactor batch fermentation with glucose as the carbon source, the recombinant E. coli B2/pED+pVio exhibited a crude violacein titer of 1.75 g L(-1) and a productivity of 36 mg L(-1) h(-1), which was the highest titer and productivity reported so far under the similar culture conditions without tryptophan addition.
conclusionMetabolic pathway analysis using 13C labeling illustrated that the up-regulated tryptophan supply enhanced tryptophan metabolism from glucose, whereas the introduction of violacein pathway drew more carbon flux from glucose to tryptophan, thereby contributing to the effective production of crude violacein in the engineered E. coli cell factory.
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