ArticleMicrobial cell factories2015
Inhibition of oxidative phosphorylation for enhancing citric acid production by Aspergillus niger.
Article in Microbial cell factories, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 47 citations in OpenAlex.
- Review
- Enhanced bafilomycin A1 production in Streptomyces Griseobrunneus via protoplast fusion combined with high-throughput screening.World journal of microbiology & biotechnology · 2025Article
- Microbial Bio-refineries: Engineering Microbes for the Production of Versatile Short-Chain Organic Acids.Indian journal of microbiology · 2025Review
- Synthetic biology meets Aspergillus: engineering strategies for next-generation organic acid production.World journal of microbiology & biotechnology · 2025Review
- Antifungal mechanism and transcriptome analysis of Bacillomycin D-C16 againstFrontiers in microbiology · 2025Article
- PemK's Arg24 is a crucial residue for PemIK toxin-antitoxin system to induce the persistence ofFrontiers in microbiology · 2024Article
- Integration of Aspergillus niger transcriptomic profile with metabolic model identifies potential targets to optimise citric acid production from lignocellulosic hydrolysate.Biotechnology for biofuels and bioproducts · 2022Article
- The importance of complete and high-quality genome sequences inFrontiers in fungal biology · 2022Article
- Enhancing erythritol production from crude glycerol in a wild-typeFrontiers in microbiology · 2022Article
- Fine-tuning mitochondrial activity in Yarrowia lipolytica for citrate overproduction.Scientific reports · 2021Article
- A control theoretic three timescale model for analyzing energy management in mammalian cancer cells.Computational and structural biotechnology journal · 2021Article
- Citric acid and itaconic acid accumulation: variations of the same story?Applied microbiology and biotechnology · 2019Review
- Systems metabolic engineering for citric acid production by Aspergillus niger in the post-genomic era.Microbial cell factories · 2019Review
- A Damaged Oxidative Phosphorylation Mechanism Is Involved in the Antifungal Activity of Citral againstFrontiers in microbiology · 2018Article
- Changing oxidoreduction potential to improve water-soluble yellow pigment production with Monascus ruber CGMCC 10910.Microbial cell factories · 2017Article
- Cloning and Characterization of a Pyruvate Carboxylase Gene from Penicillium rubens and Overexpression of the Genein the Yeast Yarrowia lipolytica for Enhanced Citric Acid Production.Marine biotechnology (New York, N.Y.) · 2016Article
- A novel strain of Yarrowia lipolytica as a platform for value-added product synthesis from glycerol.Biotechnology for biofuels · 2016Article
- Effects of heterologous expression of phosphoenolpyruvate carboxykinase and phosphoenolpyruvate carboxylase on organic acid production in Aspergillus carbonarius.Journal of industrial microbiology & biotechnology · 2015Article
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
backgroundThe spore germination rate and growth characteristics were compared between the citric acid high-yield strain Aspergillus niger CGMCC 5751 and A. niger ATCC 1015 in media containing antimycin A or DNP. We inferred that differences in citric acid yield might be due to differences in energy metabolism between these strains. To explore the impact of energy metabolism on citric acid production, the changes in intracellular ATP, NADH and NADH/NAD+ were measured at various fermentation stages. In addition, the effects of antimycin A or DNP on energy metabolism and citric acid production was investigated by CGMCC 5751.
resultsBy comparing the spore germination rate and the extent of growth on PDA plates containing antimycin A or DNP, CGMCC 5751 was shown to be more sensitive to antimycin A than ATCC 1015. The substrate-level phosphorylation of CGMCC 5751 was greater than that of ATCC 1015 on PDA plates with DNP. DNP at tested concentrations had no apparent effect on the growth of CGMCC 5751. There were no apparent effects on the mycelial morphology, the growth of mycelial pellets or the dry cell mass when 0.2 mg L(-1) antimycin A or 0.1 mg L(-1) DNP was added to medium at the 24-h time point. The concentrations of intracellular ATP, NADH and NADH/NAD+ of CGMCC 5751 were notably lower than those of ATCC 1015 at several fermentation stages. Moreover, at 96 h of fermentation, the citric acid production of CGMCC 5751 reached up to 151.67 g L(-1) and 135.78 g L(-1) by adding 0.2 mg L(-1) antimycin A or 0.1 mg L(-1) DNP, respectively, at the 24-h time point of fermentation. Thus, the citric acid production of CGMCC 5751 was increased by 19.89% and 7.32%, respectively.
conclusionsThe concentrations of intracellular ATP, NADH and NADH/NAD+ of the citric acid high-yield strain CGMCC 5751 were notably lower than those of ATCC 1015. The excessive ATP has a strong inhibitory effect on citric acid accumulation by A. niger. Increasing NADH oxidation and appropriately reducing the concentration of intracellular ATP can accelerate glycolysis and the TCA cycle to enhance citric acid yield.
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