ArticleGenome research2015
A lacZ reporter gene expression atlas for 313 adult KOMP mutant mouse lines.
Article in Genome research, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
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- The mechanosensitive ion channel PIEZO1 promotes satellite cell function in muscle regeneration.Life science alliance · 2023Article
- IRAG2 Interacts with IPInternational journal of molecular sciences · 2021Article
- Sex Differences in the Role of CNIH3 on Spatial Memory and Synaptic Plasticity.Biological psychiatry · 2021Article
- Three-Dimensional X-ray Imaging of β-Galactosidase Reporter Activity by Micro-CT: Implication for Quantitative Analysis of Gene Expression.Brain sciences · 2021Article
- Autistic-Like Behavior and Impairment of Serotonin Transporter and AMPA Receptor Trafficking inFrontiers in genetics · 2021Article
- Label-free optical imaging in developmental biology [Invited].Biomedical optics express · 2020Review
- Self-Immolative Activation of β-Galactosidase-Responsive Probes for In Vivo MR Imaging in Mouse Models.Angewandte Chemie (International ed. in English) · 2020Article
- Predicting human disease mutations and identifying drug targets from mouse gene knockout phenotyping campaigns.Disease models & mechanisms · 2019Review
- Use of gene-editing technology to introduce targeted modifications in pigs.Journal of animal science and biotechnology · 2018Review
- Germline deletion of Krüppel-like factor 14 does not increase risk of diet induced metabolic syndrome in male C57BL/6 mice.Biochimica et biophysica acta. Molecular basis of disease · 2017Article
- Loss of p73 in ependymal cells during the perinatal period leads to aqueductal stenosis.Scientific reports · 2017Article
- Disease model discovery from 3,328 gene knockouts by The International Mouse Phenotyping Consortium.Nature genetics · 2017Article
- Lysine desuccinylase SIRT5 binds to cardiolipin and regulates the electron transport chain.The Journal of biological chemistry · 2017Article
- The old and new face of craniofacial research: How animal models inform human craniofacial genetic and clinical data.Developmental biology · 2016Review
- CRISPR/Cas9-Mediated Insertion of loxP Sites in the Mouse Dock7 Gene Provides an Effective Alternative to Use of Targeted Embryonic Stem Cells.G3 (Bethesda, Md.) · 2016Article
- The Evolutionarily Conserved Tre2/Bub2/Cdc16 (TBC), Lysin Motif (LysM), Domain Catalytic (TLDc) Domain Is Neuroprotective against Oxidative Stress.The Journal of biological chemistry · 2016Article
- A gene expression resource generated by genome-wide lacZ profiling in the mouse.Disease models & mechanisms · 2015Article
- Reporter Gene Silencing in Targeted Mouse Mutants Is Associated with Promoter CpG Island Methylation.PloS one · 2015Article
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Authors and funding
11 authors.
Funding
Abstract
Expression of the bacterial beta-galactosidase reporter gene (lacZ) in the vector used for the Knockout Mouse Project (KOMP) is driven by the endogenous promoter of the target gene. In tissues from KOMP mice, histochemical staining for LacZ enzyme activity can be used to determine gene expression patterns. With this technique, we have produced a comprehensive resource of gene expression using both whole mount (WM) and frozen section (FS) LacZ staining in 313 unique KOMP mutant mouse lines. Of these, ∼ 80% of mutants showed specific staining in one or more tissues, while ∼ 20% showed no specific staining, ∼ 13% had staining in only one tissue, and ∼ 25% had staining in >6 tissues. The highest frequency of specific staining occurred in the brain (∼ 50%), male gonads (42%), and kidney (39%). The WM method was useful for rapidly identifying whole organ and some substructure staining, while the FS method often revealed substructure and cellular staining specificity. Both staining methods had >90% repeatability in biological replicates. Nonspecific LacZ staining occurs in some tissues due to the presence of bacteria or endogenous enzyme activity. However, this can be effectively distinguished from reporter gene activity by the combination of the WM and FS methods. After careful annotation, LacZ staining patterns in a high percentage of mutants revealed a unique structure-function not previously reported for many of these genes. The validation of methods for LacZ staining, annotation, and expression analysis reported here provides unique insights into the function of genes for which little is currently known.
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