ArticleJournal of visualized experiments : JoVE2015
Simple method for fluorescence DNA in situ hybridization to squashed chromosomes.
Article in Journal of visualized experiments : JoVE, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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Who cites it
46 citing papers in PubMed.
- Rapid protamine evolution suppresses meiotic drive inScience advances · 2026Article
- Chromatin state dynamics of autosomes and the B chromosome during spermatogenesis in Pseudococcus viburni.Heredity · 2026Article
- Meiotic pairing through barcode-like satellite DNA repeats.Nature communications · 2026Article
- Article
- Targeted editing of pericentromeric satellite DNA alters sensitivity to meiotic drive.bioRxiv : the preprint server for biology · 2026Article
- A male-transmitted B chromosome undergoes strong meiotic drag in females of the jewel wasp Nasonia vitripennis.PLoS biology · 2026Article
- Distinct satellite DNA composition between core and germline restricted chromosomes in Bradysia (Sciara) coprophila.G3 (Bethesda, Md.) · 2025Article
- Off-pore Nup98 condensates mobilize heterochromatic breaks and exclude Rad51.Molecular cell · 2025Article
- Transparent 3-Layered Bacterial Nanocellulose as a Multicompartment and Biomimetic Scaffold for Co-Culturing Cells.Journal of functional biomaterials · 2025Article
- Patterns of crossover distribution in Drosophila mauritiana necessitate a re-thinking of the centromere effect on crossing over.Genetics · 2025Article
- The B Chromosome of Pseudococcus viburni: A Selfish Chromosome that Exploits Whole-Genome Meiotic Drive.Genome biology and evolution · 2025Article
- "Off-pore" nucleoporins relocalize heterochromatic breaks through phase separation.bioRxiv : the preprint server for biology · 2024Article
- Sustained inactivation of the Polycomb PRC1 complex induces DNA repair defects and genomic instability in epigenetic tumors.Histochemistry and cell biology · 2024Article
- RNA polymerase II-mediated rDNA transcription mediates rDNA copy number expansion in Drosophila.PLoS genetics · 2024Article
- Sustained inactivation of the Polycomb PRC1 complex induces DNA repair defects and genomic instability in epigenetic tumors.Research square · 2024Article
- Three recent sex chromosome-to-autosome fusions in a Drosophila virilis strain with high satellite DNA content.Genetics · 2023Article
- Ribosomal DNA Instability as a Potential Cause of Karyotype Evolution.Molecular biology and evolution · 2022Article
- Review
- Article
- Defective Satellite DNA Clustering into Chromocenters Underlies Hybrid Incompatibility in Drosophila.Molecular biology and evolution · 2021Article
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Authors and funding
2 authors.
Funding
Abstract
DNA in situ hybridization (DNA ISH) is a commonly used method for mapping sequences to specific chromosome regions. This approach is particularly effective at mapping highly repetitive sequences to heterochromatic regions, where computational approaches face prohibitive challenges. Here we describe a streamlined protocol for DNA ISH that circumvents formamide washes that are standard steps in other DNA ISH protocols. Our protocol is optimized for hybridization with short single strand DNA probes that carry fluorescent dyes, which effectively mark repetitive DNA sequences within heterochromatic chromosomal regions across a number of different insect tissue types. However, applications may be extended to use with larger probes and visualization of single copy (non-repetitive) DNA sequences. We demonstrate this method by mapping several different repetitive sequences to squashed chromosomes from Drosophila melanogaster neural cells and Nasonia vitripennis spermatocytes. We show hybridization patterns for both small, commercially synthesized probes and for a larger probe for comparison. This procedure uses simple laboratory supplies and reagents, and is ideal for investigators who have little experience with performing DNA ISH.
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