ArticleJournal of visualized experiments : JoVE2014
Isolation and intravenous injection of murine bone marrow derived monocytes.
Article in Journal of visualized experiments : JoVE, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- Multifactorial effects of LGALS1 blockade sensitize tumors to immune checkpoint inhibitor.Experimental hematology & oncology · 2026Article
- Homeostatic control of energy metabolism by monocyte-derived macrophages.The EMBO journal · 2026Article
- Programmed Death Ligand-1 in Melanoma and Extracellular Vesicles Promotes Local and Regional Immune Suppression through M2-like Macrophage Polarization.The American journal of pathology · 2025Article
- Polyphenol-Nanoengineered Monocyte Biohybrids for Targeted Cardiac Repair and Immunomodulation.Advanced healthcare materials · 2025Article
- Article
- Tumor-associated macrophages promoting PD-L1 expression in infiltrating B cells through the CXCL12/CXCR4 axis in human hepatocellular carcinoma.American journal of cancer research · 2024Article
- Novel anti-inflammatory effects of the IL-1 receptor in kidney myeloid cells following ischemic AKI.Frontiers in molecular biosciences · 2024Article
- A backpack-based myeloid cell therapy for multiple sclerosis.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Cell-type-specific role of P2Y2 receptor in HDM-driven model of allergic airway inflammation.Frontiers in immunology · 2023Article
- Differential responses to immune checkpoint inhibitor dictated by pre-existing differential immune profiles in squamous cell carcinomas caused by same initial oncogenic drivers.Journal of experimental & clinical cancer research : CR · 2022Article
- The TLR-NF-kB axis contributes to the monocytic inflammatory response against a virulent strain ofFrontiers in immunology · 2022Article
- Article
- Role of hypothalamic-pituitary adrenal-axis, toll-like receptors, and macrophage polarization in pre-atherosclerotic changes induced by social isolation stress in mice.Scientific reports · 2021Article
- Inhibition of TGFβ improves hematopoietic stem cell niche and ameliorates cancer-related anemia.Stem cell research & therapy · 2021Article
- Phagocytosed Polyhedrin-Cytokine Cocrystal Nanoparticles Provide Sustained Secretion of Bioactive Cytokines from Macrophages.Biodesign research · 2021Article
- Can early treatment of twitcher mice with high dose AAVrh10-GALC eliminate the need for BMT?BioImpacts : BI · 2021Article
- Article
- Engineered immune cells as highly sensitive cancer diagnostics.Nature biotechnology · 2019Article
- Clinical improvement and enhanced collateral vessel growth after xenogenic monocyte transplantation.American journal of translational research · 2019Article
- Off-Target and Tumor-Specific Accumulation of Monocytes, Macrophages and Myeloid-Derived Suppressor Cells after Systemic Injection.Neoplasia (New York, N.Y.) · 2018Article
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Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
As a subtype of leukocytes and progenitors of macrophages, monocytes are involved in many important processes of organisms and are often the subject of various fields in biomedical science. The method described below is a simple and effective way to isolate murine monocytes from heterogeneous bone marrow. Bone marrow from the femur and tibia of Balb/c mice is harvested by flushing with phosphate buffered saline (PBS). Cell suspension is supplemented with macrophage-colony stimulating factor (M-CSF) and cultured on ultra-low attachment surfaces to avoid adhesion-triggered differentiation of monocytes. The properties and differentiation of monocytes are characterized at various intervals. Fluorescence activated cell sorting (FACS), with markers like CD11b, CD115, and F4/80, is used for phenotyping. At the end of cultivation, the suspension consists of 45%± 12% monocytes. By removing adhesive macrophages, the purity can be raised up to 86%± 6%. After the isolation, monocytes can be utilized in various ways, and one of the most effective and common methods for in vivo delivery is intravenous tail vein injection. This technique of isolation and application is important for mouse model studies, especially in the fields of inflammation or immunology. Monocytes can also be used therapeutically in mouse disease models.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.