ArticlePloS one2015
Transcriptome analysis and systemic RNAi response in the African sweetpotato weevil (Cylas puncticollis, Coleoptera, Brentidae).
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed.
- Camptothecin weakens the mechanical properties of insect cuticle by inhibiting tanning.Insect science · 2026Article
- Recent Advances in Sustainable Management ofInsects · 2026Review
- Comparative transcriptome analysis of developmental stages and characterization of core RNAi-related genes in the spittlebug Mahanarva fimbriolata.BMC genomics · 2026Article
- Insecticidal Activity ofBiology · 2025Article
- Review
- RNAi inFrontiers in plant science · 2022Article
- First Evidence of Feeding-Induced RNAi in Banana Weevil via Exogenous Application of dsRNA.Insects · 2021Article
- Transcriptome Analysis Reveals Key Genes Involved in Weevil Resistance in the Hexaploid Sweetpotato.Plants (Basel, Switzerland) · 2021Article
- RNA Interference-Based Forest Protection Products (FPPs) Against Wood-Boring Coleopterans: Hope or Hype?Frontiers in plant science · 2021Review
- Next Generation dsRNA-Based Insect Control: Success So Far and Challenges.Frontiers in plant science · 2021Review
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- First transcriptome of the Neotropical pest Euschistus heros (Hemiptera: Pentatomidae) with dissection of its siRNA machinery.Scientific reports · 2020Article
- RNAi-Mediated Suppression ofFrontiers in physiology · 2020Article
- Using de novo transcriptome assembly and analysis to study RNAi in Phenacoccus solenopsis Tinsley (Hemiptera: Pseudococcidae).Scientific reports · 2019Article
- RNA sequencing, selection of reference genes and demonstration of feeding RNAi in Thrips tabaci (Lind.) (Thysanoptera: Thripidae).BMC molecular biology · 2019Article
- The South American Fruit Fly: An Important Pest Insect With RNAi-Sensitive Larval Stages.Frontiers in physiology · 2019Article
- Induction of RNAi Core Machinery's Gene Expression by Exogenous dsRNA and the Effects of Pre-exposure to dsRNA on the Gene Silencing Efficiency in the Pea Aphid (Frontiers in physiology · 2018Article
- RNA-seq of Rice Yellow Stem BorerG3 (Bethesda, Md.) · 2017Article
- RNAi technology: a new platform for crop pest control.Physiology and molecular biology of plants : an international journal of functional plant biology · 2017Review
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Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The African sweetpotato weevil (SPW) Cylas puncticollis Boheman is one of the most important constraints of sweetpotato production in Sub-Saharan Africa and yet is largely an uncharacterized insect pest. Here, we report on the transcriptome analysis of SPW generated using an Illumina platform. More than 213 million sequencing reads were obtained and assembled into 89,599 contigs. This assembly was followed by a gene ontology annotation. Subsequently, a transcriptome search showed that the necessary RNAi components relevant to the three major RNAi pathways, were found to be expressed in SPW. To address the functionality of the RNAi mechanism in this species, dsRNA was injected into second instar larvae targeting laccase2, a gene which encodes an enzyme involved in the sclerotization of insect exoskeleton. The body of treated insects showed inhibition of sclerotization, leading eventually to death. Quantitative Real Time PCR (qPCR) confirmed this phenotype to be the result of gene silencing. Together, our results provide valuable sequence data on this important insect pest and demonstrate that a functional RNAi pathway with a strong and systemic effect is present in SPW and can further be explored as a new strategy for controlling this important pest.
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