ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2015
Primary afferent and spinal cord expression of gastrin-releasing peptide: message, protein, and antibody concerns.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 58 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
58 citing papers in PubMed, 88 citations in OpenAlex.
- Article
- Article
- The contribution of endocytic mediators to itch transmission.bioRxiv : the preprint server for biology · 2026Article
- The contribution of endocytic mediators to itch transmission.Frontiers in molecular neuroscience · 2026Article
- Neural pathways that compel us to scratch an itch.Journal of biosciences · 2024Review
- Article
- A neuropeptide code for itch.Nature reviews. Neuroscience · 2021Review
- Structures of the σNature · 2021Article
- Article
- Genetic priming of sensory neurons in mice that overexpress PAR2 enhances allergen responsiveness.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- How Do Neurons Signal Itch?Frontiers in medicine · 2021Review
- Substance P-expressing Neurons in the Superficial Dorsal Horn of the Mouse Spinal Cord: Insights into Their Functions and their Roles in Synaptic Circuits.Neuroscience · 2020Article
- [Neuropathic pruritus].Schmerz (Berlin, Germany) · 2020Article
- Neuropathic Itch.Cells · 2020Review
- Expression of green fluorescent protein defines a specific population of lamina II excitatory interneurons in the GRP::eGFP mouse.Scientific reports · 2020Article
- Physiology and Pathophysiology of Itch.Physiological reviews · 2020Review
- [Pruritus-a long journey from neurophysiology to the clinic].Der Hautarzt; Zeitschrift fur Dermatologie, Venerologie, und verwandte Gebiete · 2020Review
- Ablation of spinal cord estrogen receptor α-expressing interneurons reduces chemically induced modalities of pain and itch.The Journal of comparative neurology · 2020Article
- Central circuit mechanisms of itch.Nature communications · 2020Review
- GRP receptor and AMPA receptor cooperatively regulate itch-responsive neurons in the spinal dorsal horn.Neuropharmacology · 2020Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors at 4 institutions in 3 countries.
Funding
Abstract
There is continuing controversy relating to the primary afferent neurotransmitter that conveys itch signals to the spinal cord. Here, we investigated the DRG and spinal cord expression of the putative primary afferent-derived "itch" neurotransmitter, gastrin-releasing peptide (GRP). Using ISH, qPCR, and immunohistochemistry, we conclude that GRP is expressed abundantly in spinal cord, but not in DRG neurons. Titration of the most commonly used GRP antiserum in tissues from wild-type and GRP mutant mice indicates that the antiserum is only selective for GRP at high dilutions. Paralleling these observations, we found that a GRPeGFP transgenic reporter mouse has abundant expression in superficial dorsal horn neurons, but not in the DRG. In contrast to previous studies, neither dorsal rhizotomy nor an intrathecal injection of capsaicin, which completely eliminated spinal cord TRPV1-immunoreactive terminals, altered dorsal horn GRP immunoreactivity. Unexpectedly, however, peripheral nerve injury induced significant GRP expression in a heterogeneous population of DRG neurons. Finally, dual labeling and retrograde tracing studies showed that GRP-expressing neurons of the superficial dorsal horn are predominantly interneurons, that a small number coexpress protein kinase C gamma (PKCγ), but that none coexpress the GRP receptor (GRPR). Our studies support the view that pruritogens engage spinal cord "itch" circuits via excitatory superficial dorsal horn interneurons that express GRP and that likely target GRPR-expressing interneurons. The fact that peripheral nerve injury induced de novo GRP expression in DRG neurons points to a novel contribution of this peptide to pruritoceptive processing in neuropathic itch conditions.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.